1887

Abstract

A Gram-stain-negative, non-motile and ovoid or rod-shaped bacterial strain, DBTF-13, which was isolated from a tidal flat sediment of the Yellow Sea in South Korea, was characterized taxonomically. Strain DBTF-13 grew optimally at 25–30 °C and pH 7.0–8.0, and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain DBTF-13 formed an evolutionary lineage independent of other genera, including the genera Pseudooctadecabacter and Octadecabacter . Strain DBTF-13 exhibited 16S rRNA gene sequence similarity values of 96.9 % to the type strain of Pseudooctadecabacter jejudonensis , and of 95.8–96.5 % to the type strains of Octadecabacter species. Strain DBTF-13 contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c and C16 : 0 as the major fatty acids. The major polar lipids of strain DBTF-13 were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain DBTF-13 was 61.6 mol%. The chemotaxonomic data and other differential phenotypic properties made it reasonable to differentiate strain DBTF-13 from the genera Pseudooctadecabacter and Octadecabacter . On the basis of the data presented, strain DBTF-13 constitutes a new genus and species within the class Alphaproteobacteria , for which the name Aestuariibius insulae gen. nov., sp. nov. is proposed. The type strain is DBTF-13 (=KACC 19432=NBRC 113038).

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2018-03-05
2019-10-24
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References

  1. Yoon JH, Kang SJ, Lee SY, Oh KH, Oh TK. Seohaeicola saemankumensis gen. nov., sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2009; 59: 2675– 2679 [CrossRef] [PubMed]
    [Google Scholar]
  2. Yoon JH, Kang SJ, Jung YT, Oh TK. Gaetbulicola byunsanensis gen. nov., sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 2010; 60: 196– 199 [CrossRef] [PubMed]
    [Google Scholar]
  3. Jung YT, Kim BH, Oh TK, Yoon JH. Pseudoruegeria lutimaris sp. nov., isolated from a tidal flat sediment, and emended description of the genus Pseudoruegeria. Int J Syst Evol Microbiol 2010; 60: 1177– 1181 [CrossRef] [PubMed]
    [Google Scholar]
  4. Park S, Park DS, Bae KS, Yoon JH. Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 2014; 64: 1378– 1383 [CrossRef] [PubMed]
    [Google Scholar]
  5. Jung YT, Lee JS, Yoon JH. Maliponia aquimaris gen. nov., sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2016; 66: 2271– 2277 [CrossRef] [PubMed]
    [Google Scholar]
  6. Park S, Yoon SY, Ha MJ, Jung YT, Yoon JH. Primorskyibacter aestuariivivens sp. nov., isolated from a tidal flat, and emended description of the genus Primorskyibacter. Int J Syst Evol Microbiol 2016; 66: 5550– 5555 [CrossRef] [PubMed]
    [Google Scholar]
  7. Park S, Choi J, Choi SJ, Yoon JH. Paracoccus litorisediminis sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2017; 67: 4760– 4766 [CrossRef] [PubMed]
    [Google Scholar]
  8. Billerbeck S, Orchard J, Tindall BJ, Giebel HA, Brinkhoff T et al. Description of Octadecabacter temperatus sp. nov., isolated from the southern North Sea, emended descriptions of the genus Octadecabacter and its species and reclassification of Octadecabacter jejudonensis Park and Yoon 2014 as Pseudooctadecabacter jejudonensis gen. nov., comb. nov. Int J Syst Evol Microbiol 2015; 65: 1967– 1974 [CrossRef] [PubMed]
    [Google Scholar]
  9. Park S, Yoon JH. Octadecabacter jejudonensis sp. nov., isolated from the junction between the ocean and a freshwater spring and emended description of the genus Octadecabacter. Int J Syst Evol Microbiol 2014; 64: 719– 724 [CrossRef] [PubMed]
    [Google Scholar]
  10. Gosink JJ, Herwig RP, Staley JT. Octadecabacter arcticus gen. nov., sp. nov., and O. antarcticus, sp. nov., nonpigmented, psychrophilic gas vacuolate bacteria from polar sea ice and water. Syst Appl Microbiol 1997; 20: 356– 365 [CrossRef]
    [Google Scholar]
  11. Gosink JJ, Herwig RP, Staley JT. Octadecabacter gen. nov. In Validation of publication of new names and new combinations previously effectively published outside the IJSB, Validation List no. 64. Int J Syst Bacteriol 1998; 48: 327– 328 [Crossref]
    [Google Scholar]
  12. Kim YO, Park IS, Park S, Nam BH, Park JM et al. Octadecabacter ascidiaceicola sp. nov., isolated from a sea squirt (Halocynthia roretzi). Int J Syst Evol Microbiol 2016; 66: 296– 301 [CrossRef] [PubMed]
    [Google Scholar]
  13. Park S, Yoon SY, Jung YT, Yoon JH. Octadecabacterponticola sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2016; 66: 4179– 4184 [CrossRef] [PubMed]
    [Google Scholar]
  14. Park S, Won SM, Kim H, Park DS, Yoon JH. Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2014; 64: 2969– 2974 [CrossRef] [PubMed]
    [Google Scholar]
  15. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19: 1– 67
    [Google Scholar]
  16. Bruns A, Rohde M, Berthe-Corti L. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 2001; 51: 1997– 2006 [CrossRef] [PubMed]
    [Google Scholar]
  17. Barrow GI, Feltham RKA. Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd ed. Cambridge: Cambridge University Press; 1993; [Crossref]
    [Google Scholar]
  18. Baumann P, Baumann L. The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG et al. (editors) The Prokaryotes Berlin: Springer; 1981; pp. 1302– 1331
    [Google Scholar]
  19. Cohen-Bazire G, Sistrom WR, Stanier RY. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Comp Physiol 1957; 49: 25– 68 [CrossRef] [PubMed]
    [Google Scholar]
  20. Staley JT. Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 1968; 95: 1921– 1942 [PubMed]
    [Google Scholar]
  21. Yoon JH, Kim H, Kim SB, Kim HJ, Kim WY et al. Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 1996; 46: 502– 505 [CrossRef]
    [Google Scholar]
  22. Yoon JH, Lee ST, Kim SB, Kim WY, Goodfellow M et al. Restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains. Int J Syst Bacteriol 1997; 47: 111– 114 [CrossRef]
    [Google Scholar]
  23. Yoon JH, Kim IG, Shin DY, Kang KH, Park YH. Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 2003; 53: 53– 57 [CrossRef] [PubMed]
    [Google Scholar]
  24. Komagata K, Suzuki K. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19: 161– 207 [Crossref]
    [Google Scholar]
  25. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  26. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
  27. Embley TM, Wait R. Structural lipids of eubacteria. In Goodfellow M, O’Donnell AG. (editors) Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics Chichester: John Wiley and Sons; 1994; pp. 121– 161
    [Google Scholar]
  28. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25: 125– 128 [CrossRef]
    [Google Scholar]
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