1887

Abstract

A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterial strain, designated RA3-3-1, was isolated from splendid alfonsino () collected from the North Pacific Ocean. Strain RA3-3-1 grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 1.0–3.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain RA3-3-1 belonged to the genus , clustering with the type strain of . Strain RA3-3-1 exhibited 16S rRNA gene sequence similarities of 98.7, 97.6 and 97.3 % to the type strains of , and , respectively, and of 95.5–96.4 % to the type strains of the other species. Strain RA3-3-1 contained MK-6 as the predominant menaquinone and anteiso-C, iso-C 3-OH, summed feature 3 (C 7 and/or C 6), iso-C 3-OH, iso-C and C 2-OH as the major fatty acids. The major polar lipids detected in strain RA3-3-1 were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C content of strain RA3-3-1 was 34.1 mol% and its DNA–DNA relatedness values with the type strains of , and were 12–29 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain RA3-3-1 is separate from recognized species of the genus . On the basis of the data presented, strain RA3-3-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RA3-3-1 (=KCTC 62140=NBRC 113024).

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2018-04-01
2024-03-29
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