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Volume 68, Issue 5

sp. nov., a novel actinobacterium from fishpond sediment in Taiwan Free

Abstract

Strain 06168H-1 was isolated from a fishpond sediment sample collected from the southern area of Taiwan, and a polyphasic approach was used to determine its taxonomic position. The isolate grew between 20–40 °C and 0–8 % (w/v) NaCl. It produced branched and unfragmented substrate mycelia. Short spore chains (3–10 spores per chain) formed on branched aerial mycelia. The spore chains contained non-motile, smooth-surfaced, oval spores. Galactose, arabinose and ribose were the whole-cell sugars and -diaminopimelic acid was present in its peptidoglycan. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylcholine, hydroxyphosphati dylethanolamine and a ninhydrin-positive phosphoglycolipid. The predominant menaquinone was MK-9(H) and minor components were MK-8(H) and MK-9(H). Mycolic acids were not detected. The major cellular fatty acids were iso-C and Cω6 and Cω8. The DNA G+C content of the strain was 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed this strain clustered with the members of the genus and was closely related to and . The levels of similarity between this strain and the closely related species were: BCRC16887, 98.34 %; BCRC 16220, 98.27 %; and BCRC 16886, 97.99 %. Based on phylogenetic characteristics, strain 06168H-1 represents a novel species of the genus We thus propose the name sp. nov. for this novel strain, with strain 06168H-1 (=BCRC 16893=KCTC 19743) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinobacteria and Saccharomonospora piscinae
© 2018 IUMS
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2018-05-01
2024-04-23
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References

  1. Nonomura H, Ohara Y. Distribution of actinomycetes in soil. X. New genus and species of monosporic actinomycetes in soil. J Ferment Technol 1971; 49:895–903
     [Google Scholar]
  2. Jin X, Xu LH, Mao PH, Hseu TH, Jiang CL. Description of Saccharomonospora xinjiangensis sp. nov. based on chemical and molecular classification. Int J Syst Bacteriol 1998; 48:1095–1099 [View Article]
     [Google Scholar]
  3. Runmao H. Saccharomonospora azurea sp. nov., a new species from soil. Int J Syst Bacteriol 1987; 37:60–61 [View Article]
     [Google Scholar]
  4. Syed DG, Tang SK, Cai M, Zhi XY, Agasar D et al. Saccharomonospora saliphila sp. nov., a halophilic actinomycete from an Indian soil. Int J Syst Evol Microbiol 2008; 58:570–573 [View Article][PubMed]
     [Google Scholar]
  5. Li WJ, Tang SK, Stackebrandt E, Kroppenstedt RM, Schumann P et al. Saccharomonospora paurometabolica sp. nov., a moderately halophilic actinomycete isolated from soil in China. Int J Syst Evol Microbiol 2003; 53:1591–1594 [View Article][PubMed]
     [Google Scholar]
  6. Al-Zarban SS, Abbas I, Stackebrandt E, Kroppenstedt RM, Al-Musallam AA. Saccharomonospora halophila sp. nov., a novel halophilic actinomycete isolated from marsh soil in Kuwait. Int J Syst Evol Microbiol 2002; 52:555–558 [View Article]
     [Google Scholar]
  7. Runmao H, Lin C, Guizhen W. Saccharomonospora cyanea sp. nov. Int J Syst Bacteriol 1988; 38:444–446 [View Article]
     [Google Scholar]
  8. Greiner-Mai E, Korn-Wendisch F, Kutzner HJ. Taxonomic revision of the genus Saccharomonospora and description of Saccharomonospora glauca sp. nov. Int J Syst Bacteriol 1988; 38:398–405 [View Article]
     [Google Scholar]
  9. Li D, Chen ZJ, Luo XX, Xia ZF, Wan CX et al. Saccharomonospora xiaoerkulensis sp. nov., isolated from lake sediment. Int J Syst Evol Microbiol 2016; 66:5145–5149 [View Article][PubMed]
     [Google Scholar]
  10. Liu Z, Li Y, Zheng LQ, Huang YJ, Li WJ. Saccharomonospora marina sp. nov., isolated from an ocean sediment of the East China Sea. Int J Syst Evol Microbiol 2010; 60:1854–1857 [View Article][PubMed]
     [Google Scholar]
  11. Zhang DF, Chen W, He J, Zhang XM, Xiong ZJ et al. Saccharomonospora oceani sp. nov. isolated from marine sediments in Little Andaman, India. Antonie van Leeuwenhoek 2013; 103:1377–1384 [View Article][PubMed]
     [Google Scholar]
  12. Veyisoglu A, Sazak A, Cetin D, Guven K, Sahin N. Saccharomonospora amisosensis sp. nov., isolated from deep marine sediment. Int J Syst Evol Microbiol 2013; 63:3782–3786 [View Article][PubMed]
     [Google Scholar]
  13. Schuurmans DM, Olson BH, San Clemente CL. Production and isolation of thermoviridin, an antibiotic produced by Thermoactinomyces viridis n. sp. Appl Microbiol 1956; 4:61–66[PubMed]
     [Google Scholar]
  14. Itoh T, Kudo T, Parenti F, Seino A. Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 1989; 39:168–173 [View Article]
     [Google Scholar]
  15. Kelly KL. Inter-Society Color Council–National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office; 1964
     [Google Scholar]
  16. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966; 16:313–340 [View Article]
     [Google Scholar]
  17. Goodfellow M. Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 1971; 69:33–80 [View Article]
     [Google Scholar]
  18. Gordon RE, Barnett DA, Handerhan JE, Pang CHN. Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 1974; 24:54–63 [View Article]
     [Google Scholar]
  19. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983; 29:319–322 [View Article]
     [Google Scholar]
  20. Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M. Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 1980; 188:221–233 [View Article]
     [Google Scholar]
  21. Minnikin DE, Alshamaony L, Goodfellow M. Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 1977; 88:200–204 [View Article]
     [Google Scholar]
  22. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article]
     [Google Scholar]
  23. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 1990; 20:16
     [Google Scholar]
  24. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25:125–128 [View Article]
     [Google Scholar]
  25. Nakajima Y, Kitpreechavanich V, Suzuki KI, Kudo T. Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 1999; 49:1761–1767 [View Article]
     [Google Scholar]
  26. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  27. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425
     [Google Scholar]
  28. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article]
     [Google Scholar]
  29. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
     [Google Scholar]
  30. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  31. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
     [Google Scholar]
  32. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
     [Google Scholar]
  33. Goris J, Suzuki K, Vos PD, Nakase T, Kersters K. Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 1998; 44:1148–1153 [View Article]
     [Google Scholar]
  34. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [Crossref]
     [Google Scholar]
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Saccharomonospora piscinae sp. nov., a novel actinobacterium from fishpond sediment in Taiwan
Int J Syst Evol Microbiol 68, 1418 (2018); https://doi.org/10.1099/ijsem.0.002653
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