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Volume 68, Issue 4

sp. nov., a moderately halophilic bacterium isolated from a saline soil Free

Abstract

A novel, Gram-stain-negative, aerobic, rod-shaped, motile and moderately halophilic bacterium, designated strain LCB169, was isolated from a saline soil sample from Gansu Province, PR China. The cells of LCB169 grew at 10–52 °C (optimum 30 °C), at pH 6.0–10.0 (optimum pH 8.0) and in the presence of 0–17 % (w/v) NaCl (optimum 10–15 %). Phylogenetic analysis based on 16S rRNA gene sequences and concatenated 16S rRNA, and genes sequences revealed that LCB169 represented a member of the genus in the class . The most closely related species were DQD2-30 (98.0 % 16S rRNA gene sequence similarity), AIR-2 (97.8 %) and FB2 (97.5 %). DNA–DNA relatedness values between LCB169 and CGMCC 1.6443, DSM 9502 and DSM 17331 were 33, 35 and 38 %, respectively. The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and three unidentified phospholipids. The major fatty acids were Cω7, C, Cω7 and C 3-OH. The genomic DNA G+C content was 66.1 mol% and the predominant respiratory quinone was Q-9. On the basis of the results of phenotypic, phylogenetic and chemotaxonomic analyses and DNA–DNA hybridization relatedness values, LCB169 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LCB169 (=CGMCC 1.15818=KCTC 52618).

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Keyword(s): Halomonas saliphila , halophilic , novel species and saline soil
© 2018 IUMS
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2018-04-01
2024-04-18
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Halomonas saliphila sp. nov., a moderately halophilic bacterium isolated from a saline soil
Int J Syst Evol Microbiol 68, 1153 (2018); https://doi.org/10.1099/ijsem.0.002644
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