gen. nov., sp. nov., a new member of the phylum isolated from an oilsands tailings pond Free

Abstract

A novel member of the phylum was isolated from an oilsands tailings pond in Alberta, Canada. Cells of isolate NVT are Gram-negative, strictly aerobic, non-pigmented, non-motile cocci to diplococci 0.5–1.0 µm in diameter. The bacterium is neutrophilic (optimum pH 6.0–8.0) but alkalitolerant, capable of growth between pH 5.5 and 11.0. The temperature range for growth is 15–40 °C (optimum 25–37 °C). Carbon and energy sources include sugars and organic acids. Nitrogen sources include nitrate, urea, -glycine, -alanine, -proline and -serine. Does not fix atmospheric nitrogen. Does not require NaCl and is inhibited at NaCl concentrations above 3.0 % (w/v). The DNA G+C content of strain NVT, based on a draft genome sequence, is 66.1 mol%. MK-6 and MK-7 are the major respiratory quinones. Major cellular fatty acids are anteiso-C and iso-C. Phylogenetic analysis of 16S rRNA gene sequences revealed that the strain belongs to the family of the phylum The most closely related validated species is (93.7 % 16S rRNA gene sequence identity to its type strain PB90-1). Based on genotypic, phenotypic and chemotaxonomic characteristics, it was concluded that this strain represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of this novel species is NVT (=ATCC BAA-2697;=DSM 29249).

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2018-04-01
2024-03-29
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