sp. nov., isolated from diseased farmed Murray cod () Free

Abstract

A taxonomic study was carried out on strain LW15, which was isolated from the external lesions of diseased farmed Murray cod () from an intensive culture pond. Cells of strain LW15 were Gram-negative, facultative-anaerobic, non-motile, and both coccobacillus- and bacillus-shaped. Growth was observed at NaCl concentrations of 0–2 % (w/v) (optimum, 0 %), 4–32 °C (optimum, 25–28 °C) and pH 5.0–9.0 (optimum, 7.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LW15 was affiliated to the genus , showing the highest similarity to CIP 63.46 (97.7 %) and other species with validly published names (93.5–97.6 %). Whole-genome sequencing and phylogeny reconstruction based on a core set of 1061 genes indicated that strain LW15 was most closely related to the clade formed by CIP 63.46 and CIP 70.12 and distantly related to any of the described species of genus . Furthermore, strain LW15 could be distinguished from all known species by its ability to assimilate β-alanine and -arginine, but not -glucose. The principal fatty acids were Cω9, C and Cω7/Cω6. The major respiratory quinone was Q-9. Polar lipids of strain LW15 comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four phospholipids, aminolipid and two unknown lipids. Based on its phenotypic and genotypic data, strain LW15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LW15 (=MCCC 1K03337=CICC 24241=KCTC 62134=JCM 32101).

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2018-03-01
2024-03-29
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