1887

Abstract

Two Gram-staining-positive, strictly aerobic bacilli, designated as strains Ma50-5 and Ma50-6, were isolated from the hydrothermal sediments of Manus Basin in the western Pacific Ocean. Based on 16S rRNA gene sequence, strains Ma50-5 and Ma50-6 were most closely related to Bacillus alveayuensis (97.0 and 97.2 % identity, respectively). The 16S rRNA gene sequence identity between strains Ma50-5 and Ma50-6 was 97.4 %. The identities between strains Ma50-5 and Ma50-6 and other closely related organisms were below 97.0 %. The G+C contents of the genomic DNA of strains Ma50-5 and Ma50-6 were 43.4 and 47.6 mol%, respectively. The major fatty acids (>10 %) of both strains were iso-C15 : 0 and iso-C17 : 0. The predominant isoprenoid quinone detected in both strains was menaquinone-7. Phylogenetic, physiological, biochemical and morphological analyses suggested that strains Ma50-5 and Ma50-6 represent two novel species of the genus Bacillus , for which the names Bacillus kexueae sp. nov. (type strain Ma50-5=KCTC 33881=CCTCC AB 2017020) and Bacillus manusensis sp. nov. (type strain Ma50-6=KCTC 33882=CCTCC AB 2017019), respectively, are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002594
2018-01-24
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/3/829.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002594&mimeType=html&fmt=ahah

References

  1. Cohn F. Untersuchungen uber Bakterien. Beitr Biol Pflanz 1872; 1: 127– 224
    [Google Scholar]
  2. Logan NA, Vos DP. Genus I. Bacillus. In de Vos P, Garrity G, Jones D, Krieg NR, Ludwig W et al. (editors) Bergey’s Manual of Systematic Bacteriology New York: Springer; 2009; pp. 21– 128
    [Google Scholar]
  3. Nishida A, Miyamoto H, Horiuchi S, Watanabe R, Morita H et al. Bacillus hisashii sp. nov., isolated from the caeca of gnotobiotic mice fed with thermophile-fermented compost. Int J Syst Evol Microbiol 2015; 65: 3944– 3949 [CrossRef] [PubMed]
    [Google Scholar]
  4. Zhao F, Feng YZ, Chen RR, Zhang HY, Wang JH et al. Bacillus fengqiuensis sp. nov., isolated from a typical sandy loam soil under long-term fertilization. Int J Syst Evol Microbiol 2014; 64: 2849– 2856 [CrossRef] [PubMed]
    [Google Scholar]
  5. Sultanpuram VR, Mothe T, Chintalapati S, Chintalapati VR. Bacillus catenulatus sp. nov., an alkalitolerant bacterium isolated from a soda lake. Arch Microbiol 2017; 199: 1391– 1397 [CrossRef] [PubMed]
    [Google Scholar]
  6. Kämpfer P, Busse HJ, McInroy JA, Hu CH, Kloepper JW et al. Bacillus zeae sp. nov., isolated from the rhizosphere of Zea mays. Int J Syst Evol Microbiol 2017; 67: 1241– 1246 [CrossRef] [PubMed]
    [Google Scholar]
  7. Pal D, Mathan Kumar R, Kaur N, Kumar N, Kaur G et al. Bacillus maritimus sp. nov., a novel member of the genus Bacillus isolated from marine sediment. Int J Syst Evol Microbiol 2017; 67: 60– 66 [CrossRef] [PubMed]
    [Google Scholar]
  8. Tidjani Alou M, Rathored J, Traore SI, Khelaifia S, Michelle C et al. Bacillus niameyensis sp. nov., a new bacterial species isolated from human gut. New Microbes New Infect 2015; 8: 61– 69 [CrossRef] [PubMed]
    [Google Scholar]
  9. Guo J, Wang YQ, Yang G, Chen Y, Zhou S et al. Bacillus nitroreducens sp. nov., a humus-reducing bacterium isolated from a compost. Arch Microbiol 2016; 198: 347– 352 [CrossRef] [PubMed]
    [Google Scholar]
  10. Bae SS, Lee JH, Kim SJ. Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough. Int J Syst Evol Microbiol 2005; 55: 1211– 1215 [CrossRef] [PubMed]
    [Google Scholar]
  11. Wang YQ, Yuan Y, Yu Z, Yang GQ, Zhou SG. Bacillus borbori sp. Nov., isolated from an electrochemically active biofilm. Curr Microbiol 2013; 67: 718– 724 [CrossRef] [PubMed]
    [Google Scholar]
  12. Arfman N, Dijkhuizen L, Kirchhof G, Ludwig W, Schleifer KH et al. Bacillus methanolicus sp. nov., a new species of thermotolerant, methanol-utilizing, endospore-forming bacteria. Int J Syst Bacteriol 1992; 42: 439– 445 [CrossRef] [PubMed]
    [Google Scholar]
  13. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62: 716– 721 [CrossRef] [PubMed]
    [Google Scholar]
  14. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30: 2725– 2729 [CrossRef] [PubMed]
    [Google Scholar]
  15. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50: 1861– 1868 [CrossRef] [PubMed]
    [Google Scholar]
  16. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005; 55: 1149– 1153 [CrossRef] [PubMed]
    [Google Scholar]
  17. Logan NA, Vos PD. Bacillus. In Bergey’s Manual of Systematics of Archaea and Bacteria New Jersey: Wiley; 2015; pp. 1– 164
    [Google Scholar]
  18. Winnepenninckx B, Backeljau T, de Wachter R. Extraction of high molecular weight DNA from molluscs. Trends Genet 1993; 9: 407 [PubMed] [Crossref]
    [Google Scholar]
  19. de Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970; 12: 133– 142 [CrossRef] [PubMed]
    [Google Scholar]
  20. Huss VA, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983; 4: 184– 192 [CrossRef] [PubMed]
    [Google Scholar]
  21. Marmur J. A procedure for the isolation of deoxyribonucleic acid from microorganisms. Method Enzymol 1963; 6: 726– 738 [Crossref]
    [Google Scholar]
  22. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. USFCC News Lett 1990; 20: 1– 6
    [Google Scholar]
  23. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
  24. Pukall R, Schumann P, Clermont D, Bizet C. Bacillus aeolius DSM 15084T (=CIP 107628T) is a strain of Bacillus licheniformis. Int J Syst Evol Microbiol 2008; 58: 1268– 1270 [CrossRef] [PubMed]
    [Google Scholar]
  25. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37: 463– 464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002594
Loading
/content/journal/ijsem/10.1099/ijsem.0.002594
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error