RT Journal Article SR Electronic(1) A1 Singh, Harjodh A1 Kaur, Manpreet A1 Kaur, Lakhwinder A1 Sharma, Shivani A1 Mishra, Sunita A1 Tanuku, Naga Radha Srinivas A1 Pinnaka, Anil KumarYR 2018 T1 Bacillus lacus sp. nov., isolated from a water sample of a salt lake in India JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 3 SP 801 OP 809 DO https://doi.org/10.1099/ijsem.0.002588 PB Microbiology Society, SN 1466-5034, AB A strictly aerobic, alkaliphilic, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK74T, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India. Colonies were circular, 1.2 mm in diameter, shiny, smooth, whitish and convex with an entire margin after 48 h growth at 37 °C with pH 9.0. Growth occurred at 25–42 °C, 0–4 % (w/v) NaCl and at a pH of 7–12. Strain AK74T was positive for aesculinase, caseinase, lipase activities and negative for oxidase, catalase, amylase, cellulase, DNase, gelatinase and urease activities. The fatty acids were dominated by branched iso-, anteiso- and saturated fatty acids with a high abundance of iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1 and the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The DNA G+C content of strain AK74T was 51.6 mol%. A blast sequence similarity search based on 16S rRNA gene sequences indicated that Bacillus niabensis , Bacillus idriensis and Bacillus halosaccharovorans were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 96.6, 96.6 and 96.5%, respectively. Phylogenetic analysis showed that strain AK74T clustered with Bacillus mangrove and together clustered with Bacillus idriensis and Bacillus indicus . Based on its phenotypic characteristics and on phylogenetic inference, strain AK74T represents a novel species of the genus Bacillus , for which the name Bacillus lacus sp. nov. is proposed. The type strain is AK74T (=MTCC 12638T=KCTC 33946T=JCM 32185T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002588