1887

Abstract

A Gram-stain-negative, aerobic, motile and rod-shaped bacterium, designated MC28, was isolated from storage liquid collected from the stems of Populus euphratica in the Xinjiang province of China. The growth range of NaCl concentration was 0.5–6.0 % (w/v), with an optimum at 3.0 % (w/v), the temperature range for growth was 10–45 °C, with an optimum at 40 °C, and the pH range for growth was 6.0–9.0, with an optimum around pH 8.5. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MC28 formed a distinct lineage in the clade of genus Halomonas and is closely related to Halomonas desiderata DSM 9502 (96.4 %), Halomonas heilongjiangensis DSM 26881 (96.2 %) and Halomonas urumqiensis JCM 30202 (95.2 %). The average nucleotide identity, average amino acid identity and in silico DNA–DNA hybridization values between strain MC28 and the references strains were 77.2–80.3, 65.8–76.8 and 21.6–25.6 %, respectively. Chemotaxonomic analysis indicated that the main respiratory quinones were Q-9 and Q-8, the predominant cellular fatty acids were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 1 ω9c, C16 : 0 and C17 : 1 ω9c, the major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside, an aminophospholipid, an unidentified phospholipid, an unidentified aminolipid and two unidentified lipids. Based on its phenotypic, chemotaxonomic and phylogenetic characteristics, strain MC28 is considered to represent a novel species, for which the name Halomonas endophytica sp. nov. is proposed. The type strain is MC28 (=KCTC 52999=MCCC 1K03343).

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2018-03-21
2019-10-14
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References

  1. Vreeland RH, Litchfield CD, Martin EL, Elliot E. Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int J Syst Bacteriol 1980; 30: 485– 495 [CrossRef]
    [Google Scholar]
  2. Tang X, Zhai L, Lin Y, Yao S, Wang L et al. Halomonas alkalicola sp. nov., isolated from a household product plant. Int J Syst Evol Microbiol 2017; 67: 1546– 1550 [CrossRef] [PubMed]
    [Google Scholar]
  3. Dou G, He W, Liu H, Ma Y. Halomonas heilongjiangensis sp. nov., a novel moderately halophilic bacterium isolated from saline and alkaline soil. Antonie van Leeuwenhoek 2015; 108: 403– 413 [CrossRef] [PubMed]
    [Google Scholar]
  4. Zhang S, Pan J, Lu W, Yan Y, Wang H et al. Halomonas urumqiensis sp. nov., a moderately halophilic bacterium isolated from a saline-alkaline lake. Int J Syst Evol Microbiol 2016; 66: 1962– 1969 [CrossRef] [PubMed]
    [Google Scholar]
  5. Carlson RP, Oshota O, Shipman M, Caserta JA, Hu P et al. Integrated molecular, physiological and in silico characterization of two Halomonas isolates from industrial brine. Extremophiles 2016; 20: 261– 274 [CrossRef] [PubMed]
    [Google Scholar]
  6. Lee JC, Kim SJ, Whang KS. Halomonas sediminicola sp. nov., a moderately halophilic bacterium isolated from a solar saltern sediment. Int J Syst Evol Microbiol 2016; 66: 3865– 3872 [CrossRef] [PubMed]
    [Google Scholar]
  7. Xu L, Xu XW, Meng FX, Huo YY, Oren A et al. Halomonas zincidurans sp. nov., a heavy-metal-tolerant bacterium isolated from the deep-sea environment. Int J Syst Evol Microbiol 2013; 63: 4230– 4236 [CrossRef] [PubMed]
    [Google Scholar]
  8. Jung WY, Lee HJ, Jeon CO. Halomonas garicolasp. nov., isolated from saeu-jeot, a Korean salted and fermented shrimp sauce. Int J Syst Evol Microbiol 2016; 66: 731– 737 [CrossRef] [PubMed]
    [Google Scholar]
  9. Soto-Ramírez N, Sánchez-Porro C, Rosas S, González W, Quiñones M et al. Halomonas avicenniae sp. nov., isolated from the salty leaves of the black mangrove Avicennia germinans in Puerto Rico. Int J Syst Evol Microbiol 2007; 57: 900– 905 [CrossRef] [PubMed]
    [Google Scholar]
  10. Kim KK, Lee KC, Oh HM, Lee JS. Halomonas stevensii sp. nov., Halomonas hamiltonii sp. nov. and Halomonas johnsoniae sp. nov., isolated from a renal care centre. Int J Syst Evol Microbiol 2010; 60: 369– 377 [CrossRef] [PubMed]
    [Google Scholar]
  11. Franzmann PD, Burton HR, Mcmeekin TA. Halomonas subglaciescola, a new species of halotolerant bacteria isolated from Antarctica. Int J Syst Bacteriol 1987; 37: 27– 34 [CrossRef]
    [Google Scholar]
  12. Zhao B, Yan Y, Chen S. How could haloalkaliphilic microorganisms contribute to biotechnology?. Can J Microbiol 2014; 60: 717– 727 [CrossRef] [PubMed]
    [Google Scholar]
  13. Coronado M, Vargas C, Hofemeister J, Ventosa A, Nieto JJ. Production and biochemical characterization of an alpha-amylase from the moderate halophile Halomonas meridiana. FEMS Microbiol Lett 2000; 183: 67– 71 [PubMed]
    [Google Scholar]
  14. Poli A, Nicolaus B, Denizci AA, Yavuzturk B, Kazan D. Halomonas smyrnensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 2013; 63: 10– 18 [CrossRef] [PubMed]
    [Google Scholar]
  15. Qu L, Lai Q, Zhu F, Hong X, Zhang J et al. Halomonas daqiaonensis sp. nov., a moderately halophilic, denitrifying bacterium isolated from a littoral saltern. Int J Syst Evol Microbiol 2011; 61: 1612– 1616 [CrossRef] [PubMed]
    [Google Scholar]
  16. Gerceker D, Karasartova D, Elyürek E, Barkar S, Kiyan M et al. A new, simple, rapid test for detection of DNase activity of microorganisms: DNase tube test. J Gen Appl Microbiol 2009; 55: 291– 294 [CrossRef] [PubMed]
    [Google Scholar]
  17. Chen C, Su Y, Tao T, Fu G, Zhang C et al. Maripseudobacter aurantiacus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a sedimentation basin. Int J Syst Evol Microbiol 2017; 67: 778– 783 [CrossRef] [PubMed]
    [Google Scholar]
  18. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39: 159– 167 [CrossRef]
    [Google Scholar]
  19. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613– 1617 [CrossRef] [PubMed]
    [Google Scholar]
  20. Zhang XQ, Sun C, Wang CS, Zhang X, Zhou X et al. Sinimarinibacterium flocculans gen. nov., sp. nov., a gammaproteobacterium from offshore surface seawater. Int J Syst Evol Microbiol 2015; 65: 3541– 3546 [CrossRef] [PubMed]
    [Google Scholar]
  21. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28: 2731– 2739 [CrossRef] [PubMed]
    [Google Scholar]
  22. Simpson JT, Wong K, Jackman SD, Schein JE, Jones SJ et al. ABySS: a parallel assembler for short read sequence data. Genome Res 2009; 19: 1117– 1123 [CrossRef] [PubMed]
    [Google Scholar]
  23. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25: 1043– 1055 [CrossRef] [PubMed]
    [Google Scholar]
  24. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: An improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66: 1100– 1103 [CrossRef] [PubMed]
    [Google Scholar]
  25. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14: 60 [CrossRef] [PubMed]
    [Google Scholar]
  26. Rodriguez-R LM, Konstantinidis KT. Bypassing cultivation to identify bacterial species. Microbe Magazine 2014; 9: 111– 118 [CrossRef]
    [Google Scholar]
  27. Zhong ZP, Liu Y, Liu HC, Wang F, Zhou YG et al. Roseibium aquae sp. nov., isolated from a saline lake. Int J Syst Evol Microbiol 2014; 64: 2812– 2818 [CrossRef] [PubMed]
    [Google Scholar]
  28. Zhong ZP, Liu Y, Wang F, Zhou YG, Liu HC et al. Planktosalinus lacus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a salt lake. Int J Syst Evol Microbiol 2016; 66: 2084– 2089 [CrossRef] [PubMed]
    [Google Scholar]
  29. Dong XZCM. Determinative Manual for Routine Bacteriology Beijing: Scientific Press; 2001
    [Google Scholar]
  30. Wu YH, Cheng H, Huo YY, Jin XB, Wang CS et al. Henriciella pelagia sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2017; 67: 3020– 3025 [CrossRef] [PubMed]
    [Google Scholar]
  31. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Mircrobiol 1987; 19: 161– 207 [Crossref]
    [Google Scholar]
  32. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990; 66: 199– 202 [CrossRef]
    [Google Scholar]
  33. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106: 19126– 19131 [CrossRef] [PubMed]
    [Google Scholar]
  34. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37: 463– 464 [CrossRef]
    [Google Scholar]
  35. Konstantinidis KT, Tiedje JM. Towards a genome-based taxonomy for prokaryotes. J Bacteriol 2005; 187: 6258– 6264 [CrossRef] [PubMed]
    [Google Scholar]
  36. Berendes F, Gottschalk G, Heine-Dobbernack E, Moore ERB, Tindall BJ. Halomonas desiderata sp. nov, a new alkaliphilic, halotolerant and denitrifying bacterium isolated from a municipal sewage works. Syst Appl Microbiol 1996; 19: 158– 167 [CrossRef]
    [Google Scholar]
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