1887

Abstract

A Gram-stain-negative, aerobic, motile and rod-shaped bacterium, designated MC28, was isolated from storage liquid collected from the stems of in the Xinjiang province of China. The growth range of NaCl concentration was 0.5–6.0 % (w/v), with an optimum at 3.0 % (w/v), the temperature range for growth was 10–45 °C, with an optimum at 40 °C, and the pH range for growth was 6.0–9.0, with an optimum around pH 8.5. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MC28 formed a distinct lineage in the clade of genus and is closely related to DSM 9502 (96.4 %), DSM 26881 (96.2 %) and JCM 30202 (95.2 %). The average nucleotide identity, average amino acid identity and DNA–DNA hybridization values between strain MC28 and the references strains were 77.2–80.3, 65.8–76.8 and 21.6–25.6 %, respectively. Chemotaxonomic analysis indicated that the main respiratory quinones were Q-9 and Q-8, the predominant cellular fatty acids were summed feature 8 (Cω6 and/or Cω7), summed feature 3 (Cω7 and/or Cω6), C 9, C and C 9, the major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside, an aminophospholipid, an unidentified phospholipid, an unidentified aminolipid and two unidentified lipids. Based on its phenotypic, chemotaxonomic and phylogenetic characteristics, strain MC28 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is MC28 (=KCTC 52999=MCCC 1K03343).

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2018-05-01
2024-03-28
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