1887

Abstract

A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium, designated THG-T121, was isolated from forest soil. Growth occurred at 10–40 °C (optimum 28–30 °C), at pH 6–8 (optimum 7) and at 0–4 % NaCl (optimum 1 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121 were identified as KCTC 29134 (97.9 %), KCTC 3251 (97.3 %), KCTC 19824 (97.2 %). 16S rRNA gene sequence similarities among strain THG-T121 and other recognized species were lower than 97.0 %. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, one unidentified phospholipid, three unidentified glycolipids and one unidentified lipid. The isoprenoid quinone was menaquinone (MK-10(H)). The major fatty acids were -C, -C A, C, -C, -C and -C. The whole-cell sugars of strain THG-T121 were rhamnose, ribose, mannose and glucose. The peptidoglycan type of strain THG-T121 is A4, containing -Orn–D-Ser–L-Asp. The DNA G+C content of strain THG-T121 was 72.4 mol%. DNA–DNA hybridization values between strain THG-T121 and KCTC 29134, KCTC 3251 and KCTC 19824 were 30.2 % (27.3 %, reciprocal analysis), 28.4 %, (17.3 %) and 16.9 %, (9.3 %), respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain THG-T121 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is THG-T121 (=KACC 19191=CGMCC 4.7389).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002584
2018-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/3/788.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002584&mimeType=html&fmt=ahah

References

  1. Yi H, Schumann P, Chun J. Demequina aestuarii gen. nov., sp. nov., a novel actinomycete of the suborder Micrococcineae, and reclassification of Cellulomonas fermentans Bagnara et al. 1985 as Actinotalea fermentans gen. nov., comb. nov. Int J Syst Evol Microbiol 2007; 57:151–156 [View Article][PubMed]
    [Google Scholar]
  2. Li Y, Chen F, Dong K, Wang G. Actinotalea ferrariae sp. nov., isolated from an iron mine, and emended description of the genus Actinotalea . Int J Syst Evol Microbiol 2013; 63:3398–3403 [View Article][PubMed]
    [Google Scholar]
  3. Zhao S, Li L, Li SH, Wang HF, Hozzein WN et al. Actinotalea suaedae sp. nov., isolated from the halophyte Suaeda physophora in Xinjiang, Northwest China. Antonie van Leeuwenhoek 2015; 107:1–7 [View Article][PubMed]
    [Google Scholar]
  4. Cho H, Hamada M, Ahn JH, Weon HY, Joa JH et al. Pseudactinotalea terrae gen. nov., sp. nov., isolated from greenhouse soil, and reclassification of Actinotalea suaedae as Pseudactinotalea suaedae comb. nov. Int J Syst Evol Microbiol 2017; 67:704–709 [View Article][PubMed]
    [Google Scholar]
  5. Jin L, Ko SR, Lee CS, Ahn CY, Lee JS et al. Actinotalea caeni sp. nov., isolated from a sludge sample of a biofilm reactor. Int J Syst Evol Microbiol 2017; 67:1595–1599 [View Article][PubMed]
    [Google Scholar]
  6. Stackebrandt E, Schumann P, Prauser H. The family Cellulomonadaceae . In Martin D, Stanley F, Eugene R, Karl-Heinz S, Stackebrandt E et al. (editors) The Prokaryotes. New York: Springer; 2006 pp. 983–1001 [Crossref]
    [Google Scholar]
  7. Shi Z, Luo G, Wang G. Cellulomonas carbonis sp. nov., isolated from coal mine soil. Int J Syst Evol Microbiol 2012; 62:2004–2010 [View Article][PubMed]
    [Google Scholar]
  8. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [View Article][PubMed]
    [Google Scholar]
  9. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  10. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  11. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95–98
    [Google Scholar]
  12. Kimura M. The Neutral Theory of Molecular Evolution United Kingdom: Cambridge University Press; 1984
    [Google Scholar]
  13. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  14. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  15. Kluge AG, Farris JS. Quantitative phyletics and the evolution of anurans. Syst Biol 1969; 18:1–32 [View Article]
    [Google Scholar]
  16. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  17. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  18. Buck JD. Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 1982; 44:992–993[PubMed]
    [Google Scholar]
  19. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956; 178:703 [View Article][PubMed]
    [Google Scholar]
  20. Yan ZF, Lin P, Chu X, Kook M, Li CT et al. Aeromicrobium halotolerans sp. nov., isolated from desert soil sample. Arch Microbiol 2016; 198:423–427 [View Article][PubMed]
    [Google Scholar]
  21. Yan ZF, Trinh H, Moya G, Lin P, Li CT et al. Lysobacter rhizophilus sp. nov., isolated from rhizosphere soil of mugunghwa, the national flower of South Korea. Int J Syst Evol Microbiol 2016; 66:4754–4759 [View Article][PubMed]
    [Google Scholar]
  22. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
  23. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
    [Google Scholar]
  24. Stabili L, Gravili C, Tredici SM, Piraino S, Talà A et al. Epibiotic Vibrio luminous bacteria isolated from some hydrozoa and bryozoa species. Microb Ecol 2008; 56:625–636 [View Article][PubMed]
    [Google Scholar]
  25. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [Crossref]
    [Google Scholar]
  26. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  27. Collins MD, Jones D. Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol 1980; 48:459–470 [View Article]
    [Google Scholar]
  28. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  29. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
    [Google Scholar]
  30. Hu HY, Lim BR, Goto N, Fujie K. Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples. J Microbiol Methods 2001; 47:17–24[PubMed] [Crossref]
    [Google Scholar]
  31. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982; 5:2359–2367 [View Article]
    [Google Scholar]
  32. Schumann P. 5-Peptidoglycan structure. In Fred R, Aharon O. (editors) Methods in Microbiology. London: Academic Press; 2011 pp. 101–129
    [Google Scholar]
  33. Wheeler R, Veyrier F, Werts C, Boneca IG. Peptidoglycan and Nod Receptor. In Taniguchi NET, Hart GW, Seeberger PH, Wong CH. (editors) Glycoscience: Biology and Medicine. New York: Springer; 2015 pp. 737–747 [Crossref]
    [Google Scholar]
  34. Chen YG, Tang SK, Zhang YQ, Li ZY, Yi LB et al. Arthrobacter halodurans sp. nov., a new halotolerant bacterium isolated from sea water. Antonie van Leeuwenhoek 2009; 96:63–70 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002584
Loading
/content/journal/ijsem/10.1099/ijsem.0.002584
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error