1887

Abstract

A Gram-staining-negative, aerobic, non-motile, rod-shaped bacterium, designated as P308H10, was isolated from surface sediment of the Southern Indian Ocean. Growth occurred at 4–36 °C (optimum 20–25 °C), pH 6.0–8.5 (optimum 7.5–8.0) and in the presence of 1–8 % (w/v) NaCl (optimum 2–3 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain P308H10 lies within the clade of members of the genus Arenibacter and is closely related to Arenibacter hampyeongensis HP12 (98.0 %), Arenibacter echinorum KMM 6032 (98.4 %), Arenibacter palladensis LMG 21972 (97.9 %), Arenibacter troitsensis KMM 3674 (97.9 %) and ‘ Arenibacter algicola’ TG409 (98.1 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain P308H10 and the five reference strains were 85.9–80.6 % and 30.2–23.6 %, respectively. The major fatty acids (>10 %) of strain P308H10 were summed feature 3, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0. The major polar lipids comprised phosphatidylethanolamine, five unidentified aminolipids and four unidentified lipids. The only respiratory quinone was menaquinone-6. The genomic DNA G+C content was 38.2 mol%. On the basis of the phenotypic, phylogenetic and chemotaxonomic data presented, strain P308H10 represents a novel species of the genus Arenibacter , for which the name Arenibacter catalasegens sp. nov. is proposed. The type strain is P308H10 (=GDMCC 1.1230=KCTC 52983). An emended description of the genus Arenibacter is also proposed.

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2018-01-29
2019-12-08
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References

  1. Ivanova EP, Nedashkovskaya OI, Chun J, Lysenko AM, Frolova GM et al. Arenibacter gen. nov., new genus of the family Flavobacteriaceae and description of a new species, Arenibacter latericius sp. nov. Int J Syst Evol Microbiol 2001; 51: 1987– 1995 [CrossRef] [PubMed]
    [Google Scholar]
  2. Nedashkovskaya OI, Vancanneyt M, Cleenwerck I, Snauwaert C, Kim SB et al. Arenibacter palladensis sp. nov., a novel marine bacterium isolated from the green alga Ulva fenestrata, and emended description of the genus Arenibacter. Int J Syst Evol Microbiol 2006; 56: 155– 160 [CrossRef] [PubMed]
    [Google Scholar]
  3. Nedashkovskaya OI, Suzuki M, Vysotskii MV, Mikhailov VV. Arenibacter troitsensis sp. nov., isolated from marine bottom sediment. Int J Syst Evol Microbiol 2003; 53: 1287– 1290 [CrossRef] [PubMed]
    [Google Scholar]
  4. Sun F, Wang B, du Y, Liu X, Lai Q et al. Arenibacter nanhaiticus sp. nov., isolated from marine sediment of the South China Sea. Int J Syst Evol Microbiol 2010; 60: 78– 83 [CrossRef] [PubMed]
    [Google Scholar]
  5. Li AZ, Lin LZ, Zhang MX, Zhu HH. Arenibacter antarcticus sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2017; 67: 4601– 4605 [CrossRef] [PubMed]
    [Google Scholar]
  6. Nedashkovskaya OI, Kim SB, Han SK, Lysenko AM, Mikhailov VV et al. Arenibacter certesii sp. nov., a novel marine bacterium isolated from the green alga Ulva fenestrata. Int J Syst Evol Microbiol 2004; 54: 1173– 1176 [CrossRef] [PubMed]
    [Google Scholar]
  7. Gutierrez T, Rhodes G, Mishamandani S, Berry D, Whitman WB et al. Polycyclic aromatic hydrocarbon degradation of phytoplankton-associated Arenibacter spp. and description of Arenibacter algicola sp. nov., an aromatic hydrocarbon-degrading bacterium. Appl Environ Microbiol 2014; 80: 618– 628 [CrossRef] [PubMed]
    [Google Scholar]
  8. Nedashkovskaya OI, Kim SB, Lysenko AM, Lee KH, Bae KS et al. Arenibacter echinorum sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 2007; 57: 2655– 2659 [CrossRef] [PubMed]
    [Google Scholar]
  9. Jeong SH, Jin HM, Kim JM, Jeon CO. Arenibacter hampyeongensis sp. nov., a marine bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 2013; 63: 679– 684 [CrossRef] [PubMed]
    [Google Scholar]
  10. Wilson K. Preparation of genomic DNA from bacteria. In Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG et al. (editors) Current Protocols in Molecular Biology New York: Green Publishing and Wiley-Interscience; 1987
    [Google Scholar]
  11. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406– 425 [PubMed]
    [Google Scholar]
  12. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17: 368– 376 [CrossRef] [PubMed]
    [Google Scholar]
  13. Nei M, Kumar S. Molecular Evolution and Phylogenetics New York: Oxford University Press; 2000
    [Google Scholar]
  14. Rzhetsky A, Nei M. A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 1992; 9: 945– 967
    [Google Scholar]
  15. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106: 19126– 19131 [CrossRef] [PubMed]
    [Google Scholar]
  16. Huss VA, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983; 4: 184– 192 [CrossRef] [PubMed]
    [Google Scholar]
  17. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37: 463– 464 [CrossRef]
    [Google Scholar]
  18. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50: 1861– 1868 [CrossRef] [PubMed]
    [Google Scholar]
  19. Christakis GB, Perlorentzou SP, Chalkiopoulou I, Athanasiou A, Legakis NJ. Chryseobacterium indologenes non-catheter-related bacteremia in a patient with a solid tumor. J Clin Microbiol 2005; 43: 2021– 2023 [CrossRef] [PubMed]
    [Google Scholar]
  20. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: American Society of Microbiology; 2007; pp. 330– 393
    [Google Scholar]
  21. Lanyi B. Classical and rapid identification methods for medically important bacteria. Methods in microbiology 1988; 19: 1– 67 [Crossref]
    [Google Scholar]
  22. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
  23. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100: 221– 230 [CrossRef] [PubMed]
    [Google Scholar]
  24. Hiraishi A, Ueda Y, Ishihara J, Mori T. Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 1996; 42: 457– 469 [CrossRef]
    [Google Scholar]
  25. Montero-Calasanz MC, Göker M, Rohde M, Spröer C, Schumann P et al. Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium. Int J Syst Evol Microbiol 2013; 63: 4386– 4395 [CrossRef] [PubMed]
    [Google Scholar]
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