1887

Abstract

A Gram-reaction-negative, aerobic, motile by gliding, non-spore-forming, rod-shaped bacterial strain, designated 122CH820-2, was isolated from spongin. This bacterium was characterized to determine its taxonomic position by using a polyphasic approach. Strain 122CH820-2 grew well at 25–30 °C on marine agar. On the basis of 16S rRNA gene sequence similarity, strain 122CH820-2 belonged to the family Flavobacteriaceae and was closely related to Aquimarina mytili PSC33 (96.8 % sequence similarity) and A. penaei P3-1 (96.7 %). Lower sequence similarities (<96.5 %) were found with all of the other recognized members of the genus Aquimarina . The G+C content of the genomic DNA was 35.2 mol%. The major respiratory quinone was menaquinone MK-6 and the major fatty acids were C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0 3-OH. The polar lipids were phosphatidylethanolamine, one aminophospholipid and five unidentified polar lipids. Strain 122CH820-2 could be differentiated genotypically and phenotypically from recognized species of the genus Aquimarina . The isolate therefore represents a novel species, for which the name Aquimarina spongiicola sp. nov. is proposed, with the type strain 122CH820-2 (=KACC 19274=LMG 30078).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002575
2018-02-14
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/4/990.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002575&mimeType=html&fmt=ahah

References

  1. Nedashkovskaya OI, Kim SB, Lysenko AM, Frolova GM, Mikhailov VV et al. Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [Cytophaga] latercula Lewin 1969 as Stanierella latercula gen. nov., comb. nov. Int J Syst Evol Microbiol 2005; 55: 225– 229 [CrossRef] [PubMed]
    [Google Scholar]
  2. Euzéby JP. List of bacterial names with standing in nomenclature: a folder available on the internet. Int J Syst Bacteriol 1997; 47: 590– 592 [CrossRef] [PubMed]
    [Google Scholar]
  3. Yu T, Zhang Z, Fan X, Shi X, Zhang XH. Aquimarina megaterium sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2014; 64: 122– 127 [CrossRef] [PubMed]
    [Google Scholar]
  4. Li G, Lai Q, Sun F, Liu X, Xie Y et al. Aquimarina atlantica sp. nov., isolated from surface seawater of the Atlantic Ocean. Antonie van Leeuwenhoek 2014; 106: 293– 300 [CrossRef] [PubMed]
    [Google Scholar]
  5. Zhang Z, Yu T, Xu T, Zhang XH. Aquimarina pacifica sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2014; 64: 1991– 1997 [CrossRef] [PubMed]
    [Google Scholar]
  6. Kennedy J, Margassery LM, O'Leary ND, O'Gara F, Morrissey J et al. Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum. Int J Syst Evol Microbiol 2014; 64: 501– 505 [CrossRef] [PubMed]
    [Google Scholar]
  7. Li X, Wang L, Huang H, Lai Q, Shao Z. Aquimarina penaei sp. nov., isolated from intestinal tract contents of Pacific white shrimp, Penaeus vannamei. Antonie van Leeuwenhoek 2014; 106: 1223– 1229 [CrossRef] [PubMed]
    [Google Scholar]
  8. Zhou YX, Wang C, du ZJ, Chen GJ. Aquimarina agarivorans sp. nov., a genome-sequenced member of the class Flavobacteriia isolated from Gelidium amansii. Int J Syst Evol Microbiol 2015; 65: 2684– 2688 [CrossRef] [PubMed]
    [Google Scholar]
  9. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173: 697– 703 [CrossRef] [PubMed]
    [Google Scholar]
  10. Kim JK, Kang MS, Park SC, Kim KM, Choi K et al. Sphingosinicella ginsenosidimutans sp. nov., with ginsenoside converting activity. J Microbiol 2015; 53: 435– 441 [CrossRef] [PubMed]
    [Google Scholar]
  11. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613– 1617 [CrossRef] [PubMed]
    [Google Scholar]
  12. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25: 4876– 4882 [CrossRef] [PubMed]
    [Google Scholar]
  13. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 1999; 41: 95– 98
    [Google Scholar]
  14. Kimura M. The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; 1983; [Crossref]
    [Google Scholar]
  15. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20: 406– 416 [CrossRef]
    [Google Scholar]
  16. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406– 425 [PubMed]
    [Google Scholar]
  17. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30: 2725– 2729 [CrossRef] [PubMed]
    [Google Scholar]
  18. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39: 783– 791 [CrossRef] [PubMed]
    [Google Scholar]
  19. Buck JD. Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 1982; 44: 992– 993 [PubMed]
    [Google Scholar]
  20. Cappuccino JG, Sherman N. Microbiology: A Laboratory Manual, 6th ed. Pearson Education, Inc.: Benjamin Cummings, CA; 2002
    [Google Scholar]
  21. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52: 1049– 1070 [CrossRef] [PubMed]
    [Google Scholar]
  22. Atlas RM. Handbook of Microbiological Media Parks LC. (editor) Boca Raton, FL: CRC Press; 1993
    [Google Scholar]
  23. Cowan ST, Steel KJ. Manual for the Identification of Medical Bacteria Cambridge: Cambridge University Press; 1974
    [Google Scholar]
  24. Ten LN, Im WT, Kim MK, Kang MS, Lee ST. Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. J Microbiol Methods 2004; 56: 375– 382 [CrossRef] [PubMed]
    [Google Scholar]
  25. Moore DD, Dowhan D. Preparation and analysis of DNA. In Ausubel FW, Brent R, Kingston RE, Moore DD, Seidman JG et al. (editors) Current Protocols in Molecular Biology New York: Wiley; 1995; pp. 2– 11
    [Google Scholar]
  26. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39: 159– 167 [CrossRef]
    [Google Scholar]
  27. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  28. Hiraishi A, Ueda Y, Ishihara J, Mori T. Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 1996; 42: 457– 469 [CrossRef]
    [Google Scholar]
  29. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
  30. Park SC, Choe HN, Baik KS, Seong CN. Aquimarina mytili sp. nov., isolated from the gut microflora of a mussel, Mytilus coruscus, and emended description of Aquimarina macrocephali. Int J Syst Evol Microbiol 2012; 62: 1974– 1979 [CrossRef] [PubMed]
    [Google Scholar]
  31. Chen WM, Sheu FS, Sheu SY. Aquimarina salinaria sp. nov., a novel algicidal bacterium isolated from a saltpan. Arch Microbiol 2012; 194: 103– 112 [CrossRef] [PubMed]
    [Google Scholar]
  32. Yoon BJ, You HS, Lee DH, Oh DC. Aquimarina spongiae sp. nov., isolated from marine sponge Halichondria oshoro. Int J Syst Evol Microbiol 2011; 61: 417– 421 [CrossRef] [PubMed]
    [Google Scholar]
  33. Park SC, Choe HN, Baik KS, Seong CN. Aquimarina gracilis sp. nov., isolated from the gut microflora of a mussel, Mytilus coruscus, and emended description of Aquimarina spongiae. Int J Syst Evol Microbiol 2013; 63: 1782– 1787 [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002575
Loading
/content/journal/ijsem/10.1099/ijsem.0.002575
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error