1887

Abstract

A heterotrophic, Gram-stain-negative, aerobic, sodium-requiring and motile bacterium was isolated from oil-contaminated surface water of the Gulf of Mexico during the Deepwater Horizon oil spill. Strain O3.65 showed highest 16S rRNA gene sequence similarity to BS107 and T5, both with 98.3 %, respectively. Based on complete genome analysis, highest similarity was observed to species of the genus . Strain O3.65 exhibited a broad salinity, temperature and pH range of 0.5–10 % NaCl, 4–45 °C and 5.5–9.0, respectively. The DNA G+C content of strain O3.65 was 61.5 mol%. The major respiratory lipoquinone was ubiquinone-10 (Q-10), the most dominant fatty acids (>1 %) comprised 18 : 1ω7 and 18 : 1ω7 11-methyl, 10 : 0 3OH, 12 : 1 3OH, 14 : 1 3OH/3-oxo-14 : 0, 16 : 0, 16 : 0 2OH, 18 : 1 2OH and 12 : 1. The polar lipid pattern indicated presence of phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid, two unidentified phospholipids and seven unidentified lipids. On Difco marine broth agar, strain O3.65 formed smooth, shiny white to beige and convex colonies with regular edges. Phylogenetic, phylogenomic and phenotypic differences revealed that strain O3.65 represents a new species of a novel genus within the family , for which we propose the name gen. nov., sp. nov. The type strain of the type species is O3.65 (=DSM 101689=LMG 29740).

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2018-03-01
2024-03-29
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