RT Journal Article SR Electronic(1) A1 Yan, Zheng-Fei A1 Lin, Pei A1 Won, Kyung-Hwa A1 Li, Chang-Tian A1 Park, GyungSoo A1 Chin, ByungSun A1 Kook, MooChang A1 Wang, Qi-Jun A1 Yi, Tae-HooYR 2018 T1 Sphingomonas rhizophila sp. nov., isolated from rhizosphere of Hibiscus syriacus JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 2 SP 681 OP 686 DO https://doi.org/10.1099/ijsem.0.002566 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, aerobic, non-motile, rod-shaped, catalase-positive and oxidase-positive bacteria (THG-T61T), was isolated from rhizosphere of Hibiscus syriacus. Growth occurred at 10–37 °C (optimum 25–30 °C), at pH 5.0–9.0 (optimum 7.0) and in the presence of 0–2.0 % NaCl (optimum without NaCl supplement). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T61T were identified as Sphingomonas ginsengisoli KCTC 12630T (97.9 %), Sphingomonas jaspsi DSM 18422T (97.8 %), Sphingomonas astaxanthinifaciens NBRC 102146T (97.4 %), Sphingomonas sediminicola KCTC 12629T (97.2 %), ‘ Sphingomonas swuensis’ KCTC 12336 (97.1 %) and Sphingomonas daechungensis KCTC 23718T (96.9 %). The isoprenoid quinone was ubiquinone-10 (Q-10). The major fatty acids were C16 : 0, C17 : 1 ω6c, summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1ω7c) and summed feature 7 (C18 : 1 ω7c, C18 : 1 ω9t and/or C18 : 1 ω12t). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, one unidentified lipid, one unidentified phospholipid, one unidentified glycolipid and one unidentified phosphoglycolipid. The polyamine was homospermidine. The DNA G+C content of strain THG-T61T was 65.6 mol%. The DNA–DNA relatedness values between strain THG-T61T and its closest reference strains were less than 49.2 %, which is lower than the threshold value of 70 %. Therefore, strain THG-T61T represents a novel species of the genus Sphingomonas , for which the name Sphingomonas rhizophila sp. nov. is proposed. The type strain is THG-T61T (=KACC 19189T=CCTCC AB 2016245T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002566