1887

Abstract

Two dark pink pigmented bacterial strains (M3 and M11) were isolated from crude oil contaminated desert sand from Kuwait. Both strains were Gram-stain-negative and small-rod to oval-shaped bacteria. Strains M3 and M11 grew at 13–42 °C (optimum, 30–35 °C) and pH 6.5–9.0 (optimum, 7.0–7.5). No additional NaCl was required for the growth of both strains. The genomic DNA G+C content of strains M3 and M11 were 69.5 and 69.0 mol%, respectively. Both strains were closely related and the mean DNA–DNA hybridization value was 92±1 %. 16S rRNA gene sequence comparisons of both strains indicated that they belong to the genus Roseomonas . Strains M3 and M11 had a sequence similarity of 97.3 and 97.4 % with Roseomonas oryzae JC288, respectively. Both strains had <97 % 16S rRNA gene sequence similarity with other members of the genus Roseomonas . Strain M3 showed 18±2 and 13±2 % reassociation (based on DNA–DNA hybridization) with R. oryzae KCTC 42542 and Roseomonas cervicalis KACC 11686, respectively. The major cellular fatty acids (>5 %) were identified as C18 : 1ω6c/C18 : 1ω7c, C16 : 1ω6c/C16 : 1ω7c and C16 : 0 in both strains. Both strains showed diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl-ethanolamine, phosphatidylcholine and unidentified glycolipid as major polar lipids. Based on distinct phenotypic, genotypic and phylogenetic differences from the previously described taxa, we propose the classification of strains M3 and M11 as representative of a novel species in the genus Roseomonas , for which the name Roseomonas deserti sp. nov. is suggested. The type strain is M3 (=KEMB 2255-459=JCM 31275).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002565
2018-01-08
2019-10-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/2/675.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002565&mimeType=html&fmt=ahah

References

  1. Weyant RS, Whitney AM. Genus Roseomonas. In Brenner DJ, Krieg NR, Staley JT. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed.vol. 2 Part C USA: Springer Science and Business Media; 2006; pp.88–92
    [Google Scholar]
  2. Venkata Ramana V, Sasikala C, Takaichi S, Ramana C. Roseomonas aestuarii sp. nov., a bacteriochlorophyll-a containing alphaproteobacterium isolated from an estuarine habitat of India. Syst Appl Microbiol 2010;33:198–203 [CrossRef][PubMed]
    [Google Scholar]
  3. Damtab J, Nutaratat P, Boontham W, Srisuk N, Duangmal K et al. Roseomonas elaeocarpi sp. nov., isolated from olive (Elaeocarpus hygrophilus Kurz.) phyllosphere. Int J Syst Evol Microbiol 2016;66:474–480 [CrossRef][PubMed]
    [Google Scholar]
  4. Wang C, Deng S, Liu X, Yao L, Shi C et al. Roseomonas eburnea sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 2016;66:385–390 [CrossRef][PubMed]
    [Google Scholar]
  5. Chu CW, Chen Q, Wang CH, Wang HM, Sun ZG et al. Roseomonas chloroacetimidivorans sp. nov., a chloroacetamide herbicide-degrading bacterium isolated from activated sludge. Antonie van Leeuwenhoek 2016;109:611–618 [CrossRef][PubMed]
    [Google Scholar]
  6. Subhash Y, Bang JJ, You TH, Lee SS. Roseomonas rubra sp. nov., isolated from lagoon sediments. Int J Syst Evol Microbiol 2016;66:3821–3827 [CrossRef][PubMed]
    [Google Scholar]
  7. Ramaprasad EV, Sasikala C, Ramana C. Roseomonas oryzae sp. nov., isolated from paddy rhizosphere soil. Int J Syst Evol Microbiol 2015;65:3535–3540 [CrossRef][PubMed]
    [Google Scholar]
  8. Kim DU, Ka JO. Roseomonas soli sp. nov., isolated from an agricultural soil cultivated with Chinese cabbage (Brassica campestris). Int J Syst Evol Microbiol 2014;64:1024–1029 [CrossRef][PubMed]
    [Google Scholar]
  9. Chen Q, Sun LN, Zhang XX, He J, Kwon SW et al. Roseomonas rhizosphaerae sp. nov., a triazophos-degrading bacterium isolated from soil. Int J Syst Evol Microbiol 2014;64:1127–1133 [CrossRef][PubMed]
    [Google Scholar]
  10. Dong L, Ming H, Yin YR, Duan YY, Zhou EM et al. Roseomonas alkaliterrae sp. nov., isolated from an alkali geothermal soil sample in Tengchong, Yunnan, South-West China. Antonie van Leeuwenhoek 2014;105:899–905 [CrossRef][PubMed]
    [Google Scholar]
  11. Kim SJ, Weon HY, Ahn JH, Hong SB, Seok SJ et al. Roseomonas aerophila sp. nov., isolated from air. Int J Syst Evol Microbiol 2013;63:2334–2337 [CrossRef][PubMed]
    [Google Scholar]
  12. Baik KS, Park SC, Choe HN, Kim SN, Moon JH et al. Roseomonas riguiloci sp. nov., isolated from wetland freshwater. Int J Syst Evol Microbiol 2012;62:3024–3029 [CrossRef][PubMed]
    [Google Scholar]
  13. Nutaratat P, Srisuk N, Duangmal K, Yurimoto H, Sakai Y et al. Roseomonas musae sp. nov., a new bacterium isolated from a banana phyllosphere. Antonie van Leeuwenhoek 2013;103:617–624 [CrossRef][PubMed]
    [Google Scholar]
  14. Kim HS, Srinivasan S, Lee SS. Methyloterrigena soli gen. nov., sp. nov., a methanol-utilizing bacterium isolated from chloroethylene-contaminated soil. Int J Syst Evol Microbiol 2016;66:101–106 [CrossRef][PubMed]
    [Google Scholar]
  15. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  16. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S et al. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  17. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  18. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  19. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961;3:208–218 [CrossRef]
    [Google Scholar]
  20. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  21. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989;39:224–229 [CrossRef]
    [Google Scholar]
  22. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
  23. Cappuccino JG, Sherman N. Microbiology - a Laboratory Manual, 8th ed. San Francisco, CA: Pearson Benjamin Cummings; 2008
    [Google Scholar]
  24. Barker J, Maxted H. Observations on the growth and movement of Acinetobacter on semi-solid media. J Med Microbiol 1975;8:443–446 [CrossRef][PubMed]
    [Google Scholar]
  25. Subhash Y, Park MJ, Lee SS. Microvirgula curvata sp. nov., isolated from hydrocarbon-contaminated soil, and emended description of the genus Microvirgula. Int J Syst Evol Microbiol 2016;66:5309–5313 [CrossRef][PubMed]
    [Google Scholar]
  26. Bushnell LD, Haas HF. The utilization of certain hydrocarbons by microorganisms. J Bacteriol 1941;41:653–673[PubMed]
    [Google Scholar]
  27. Subhash Y, Tushar L, Sasikala C, Ramana C. Falsirhodobacter halotolerans gen. nov., sp. nov., isolated from dry soils of a solar saltern. Int J Syst Evol Microbiol 2013;63:2132–2137 [CrossRef][PubMed]
    [Google Scholar]
  28. Subhash Y, Sasikala C, Ramana C. Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field. Int J Syst Evol Microbiol 2013;63:3463–3469 [CrossRef][PubMed]
    [Google Scholar]
  29. Subhash Y, Tushar L, Sasikala C, Ramana C. Erythrobacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern. Int J Syst Evol Microbiol 2013;63:4524–4532 [CrossRef][PubMed]
    [Google Scholar]
  30. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  31. Oren A, Duker S, Ritter S. The polar lipid composition of walsby's square bacterium. FEMS Microbiol Lett 1996;138:135–140 [CrossRef]
    [Google Scholar]
  32. Hiraishi A, Hoshino Y, Kitamura H. Isoprenoid quinone composition in the classification of Rhodospirillaceae. J Gen Appl Microbiol 1984;30:197–210 [CrossRef]
    [Google Scholar]
  33. Busse J, Auling G. Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 1988;11:1–8 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002565
Loading
/content/journal/ijsem/10.1099/ijsem.0.002565
Loading

Data & Media loading...

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error