@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002561, author = "Bae, Seung Seob and Jung, Jaejoon and Chung, Dawoon and Baek, Kyunghwa", title = "Marinobacterium aestuarii sp. nov., a benzene-degrading marine bacterium isolated from estuary sediment", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "2", pages = "651-656", doi = "https://doi.org/10.1099/ijsem.0.002561", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002561", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Marinobacterium", keywords = "benzene", keywords = "biodegradation", keywords = "estuary", abstract = "A Gram-stain-negative, aerobic, motile, flagellated rod-shaped bacterium, designated ST58-10T, was isolated from an estuarine sediment in the Republic of Korea. The strain was able to degrade benzene. Growth of strain ST58-10T was observed at 4–35 °C (optimum, 20–25 °C), pH 5–9 (optimum, pH 7–8) and 1–8 % NaCl (optimum, 3 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ST58-10T formed a phyletic lineage within the genus Marinobacterium of the family Oceanospirillaceae . Strain ST58-10T was most closely related to Marinobacterium profundum PAMC 27536T (99.6 %) and Marinobacterium rhizophilum CL-YJ9T (98.3 %), and to other members of the genus Marinobacterium (94.5–91.5 %). However, the mean value estimated by using the Genome-to-Genome Distance Calculator was 50.6±7.4 % with M. profundum PAMC 27536T and 30.9±2.8 with M. rhizophilum CL-YJ9T, respectively. An average nucleotide identity value was 89.0 % with M. profundum PAMC 27536T and 85.6 % with M. rhizophilum CL-YJ9T, respectively. The major fatty acids of strain ST58-10T were summed feature 3 (comprising C16 : 1 ω7c/C16 : 1 ω6c), summed feature 8 (comprising C18 : 1  ω7c/C18 : 1 ω6c), C16 : 0 and C10 : 0 3-OH, and contained ubiquinone (Q-8) as the sole isoprenoid quinone. Phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminolipids, an unidentified glycolipid and an unidentified lipid were detected as polar lipids. The DNA G+C content of strain ST58-10T was 58.78 mol%. On the basis of the phenotypic, chemotaxonomic and molecular properties, strain ST58-10T represents a novel species of the genus Marinobacterium , for which the name Marinobacterium aestuarii sp. nov. is proposed. The type strain is ST58-10T (=KCTC 52193T=NBRC 112103T).", }