1887

Abstract

During a study of bacterial diversity of soil, a novel strain, CA-15, was isolated from Kyonggi University forest soil. Cells were aerobic, Gram-stain-negative, motile, non-spore-forming, rod-shaped, oxidase-positive and catalase- negative. Tyrosine was not oxidized but produced red pigmentation on an agar palte. Strain CA-15 hydrolysed Tween 60 and DNA. It grew at 15–35 °C (optimum, 25–30 °C), pH 6.0–10.0 (optimum, 7.0–9.0) and at 1.5 % (w/v) NaCl concentration. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain CA-15 formed a lineage within the family of the class that was distinct from various species of the genus DSM 7126 was the closest member of strain CA-15 on the basis of 16S rRNA gene sequence similarity (98.48 %). Q-10 was only an isoprenoid quinone detected for strain CA-15. The major polar lipids were 1,2-di--acyl-3--[-glucopyranosyl-(1→4)- -glucopyranuronosyl]glycerol, 1,2-di--acyl-3--[ -glucopyranosyl]-sn-glycerol, 1,2-di--acyl-3- -glucopyranuronosylglycerol, 1,2-diacyl-3--[6′-phosphatidyl- -glucopyranosyl]glycerol and phosphatidylglycerol. The major cellular fatty acids were summed feature 8 (Cω7 and/or Cω6), C, Cω7 11-methyl and Cω8. The DNA G+C content of strain CA-15 was 63.6 mol%. The polyphasic characterization indicated that strain CA-15 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain of is CA-15 (=KEMB 9005-528=KACC 19106=NBRC 112677).

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2018-03-01
2020-01-27
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