1887

Abstract

A Gram-stain-negative, yellow-pigmented, non-flagellated, gliding, rod-shaped, oxidase-negative and catalase-positive bacterium, designated SE14, was isolated from soil on King George Island, South Shetland Islands, Antarctica. Strain SE14 grew at 4–25 °C (optimum, 20 °C), at pH 6.0–9.0 (optimum, pH 7.0–7.5) and with 0–3.0 % NaCl (optimum, 1.0–1.5 %), and could not produce flexirubin-type pigments. 16S rRNA gene sequence analysis showed the the isolate belonged to the genus . Strain SE14 had the highest 16S rRNA gene sequence similarity to , and with 95.8, 95.5 and 95.2 %, respectively. The strain SE14 consisted of a clade with (16S rRNA gene sequence similarity 94.9 %) and (16S rRNA gene sequence similarity 94.2 %) and simultaneously formed a distinct phyletic lineage in the neighbour-joining phylogenetic tree. Polar lipids of the strain included phosphatidylethanolamine and four unidentified aminolipids. Strain SE14 contained anteiso-C, iso-C and a mixture of iso-C 2-OH and/or C 7 as the main fatty acids, and the only respiratory quinone was menaquinone-6. The genomic DNA G+C content was 42.3 mol%. The polyphasic taxonomic study revealed that strain SE14 belongs to a novel species within the genus , and the name sp. nov. is proposed. The type strain is SE14 (=CCTCC AB 2017225=KCTC 52612).

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2018-02-01
2021-07-26
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References

  1. Bergey DH, Harrison FC, Breed RS, Hammer BW, Fm H et al. Bergey's Manual of Determinative Bacteriology Baltimore: Williams & Wilkins; 1923
    [Google Scholar]
  2. Holmes B, Owen R. Proposal that Flavobacterium breve be substituted as the type species of the genus in place of Flavobacterium aquatile and emended description of the genus Flavobacterium . Int J Syst Evol Microbiol 1979; 29:416–426
    [Google Scholar]
  3. Bernardet JF, Segers P, Vancanneyt M, Berthe F, Kersters K et al. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 1996; 46:128–148 [View Article]
    [Google Scholar]
  4. Dong K, Chen F, Du Y, Wang G. Flavobacterium enshiense sp. nov., isolated from soil, and emended descriptions of the genus Flavobacterium and Flavobacterium cauense, Flavobacterium saliperosum and Flavobacterium suncheonense . Int J Syst Evol Microbiol 2013; 63:886–892 [View Article][PubMed]
    [Google Scholar]
  5. Kang JY, Chun J, Jahng KY. Flavobacterium aciduliphilum sp. nov., isolated from freshwater, and emended description of the genus Flavobacterium . Int J Syst Evol Microbiol 2013; 63:1633–1638 [View Article][PubMed]
    [Google Scholar]
  6. Kuo I, Saw J, Kapan DD, Christensen S, Kaneshiro KY et al. Flavobacterium akiainvivens sp. nov., from decaying wood of Wikstroemia oahuensis, Hawai'i, and emended description of the genus Flavobacterium . Int J Syst Evol Microbiol 2013; 63:3280–3286 [View Article][PubMed]
    [Google Scholar]
  7. Hwang WM, Kim D, Kang K, Ahn TY. Flavobacterium eburneum sp. nov., isolated from reclaimed saline land soil. Int J Syst Evol Microbiol 2017; 67:55–59 [View Article][PubMed]
    [Google Scholar]
  8. Zhang G, Xian W, Chu Q, Yang J, Liu W et al. Flavobacterium terriphilum sp. nov., isolated from soil. Int J Syst Evol Microbiol 2016; 66:4276–4281 [View Article][PubMed]
    [Google Scholar]
  9. Suwannachart C, Rueangyotchanthana K, Srichuay S, Pheng S, Fungsin B et al. Flavobacterium tistrianum sp. nov., a gliding bacterium isolated from soil. Int J Syst Evol Microbiol 2016; 66:2241–2246 [View Article][PubMed]
    [Google Scholar]
  10. Ngo HT, Kook M, Yi TH. Flavobacterium daemonensis sp. nov., isolated from Daemo Mountain soil. Int J Syst Evol Microbiol 2015; 65:983–989 [View Article][PubMed]
    [Google Scholar]
  11. Park M, Joung Y, Nam GG, Kim S, Cho JC. Flavobacterium inkyongense sp. nov., isolated from an artificial freshwater pond. Int J Syst Evol Microbiol 2017; 67:82–86 [View Article][PubMed]
    [Google Scholar]
  12. Li AH, Liu HC, Zhou YG. Flavobacterium orientale sp. nov., isolated from lake water. Int J Syst Evol Microbiol 2017; 67:108–112 [View Article][PubMed]
    [Google Scholar]
  13. Shin SK, Ha Y, Cho YJ, Kwon S, Yong D et al. Flavobacterium gilvum sp. nov., isolated from stream water. Int J Syst Evol Microbiol 2016 [View Article][PubMed]
    [Google Scholar]
  14. Sheu SY, Chen YL, Chen WM. Flavobacterium verecundum sp. nov., isolated from a freshwater river. Int J Syst Evol Microbiol 2016; 66:3337–3344 [View Article][PubMed]
    [Google Scholar]
  15. Ao L, Zeng XC, Nie Y, Mu Y, Zhou L et al. Flavobacterium arsenatis sp. nov., a novel arsenic-resistant bacterium from high-arsenic sediment. Int J Syst Evol Microbiol 2014; 64:3369–3374 [View Article][PubMed]
    [Google Scholar]
  16. Lee K, Park SC, Yi H, Chun J. Flavobacterium limnosediminis sp. nov., isolated from sediment of a freshwater lake. Int J Syst Evol Microbiol 2013; 63:4784–4789 [View Article][PubMed]
    [Google Scholar]
  17. Kaur I, Kaur C, Khan F, Mayilraj S. Flavobacterium rakeshii sp. nov., isolated from marine sediment, and emended description of Flavobacterium beibuense Fu et al. 2011. Int J Syst Evol Microbiol 2012; 62:2897–2902 [View Article][PubMed]
    [Google Scholar]
  18. Zhang Y, Jiang F, Chang X, Qiu X, Ren L et al. Flavobacterium collinsense sp. nov., isolated from a till sample of an Antarctic glacier. Int J Syst Evol Microbiol 2016; 66:172–177 [View Article][PubMed]
    [Google Scholar]
  19. Kim JH, Choi BH, Jo M, Kim SC, Lee PC. Flavobacterium faecale sp. nov., an agarase-producing species isolated from stools of Antarctic penguins. Int J Syst Evol Microbiol 2014; 64:2884–2890 [View Article][PubMed]
    [Google Scholar]
  20. Yi H, Chun J. Flavobacterium weaverense sp. nov. and Flavobacterium segetis sp. nov., novel psychrophiles isolated from the Antarctic. Int J Syst Evol Microbiol 2006; 56:1239–1244 [View Article][PubMed]
    [Google Scholar]
  21. Yi H, Oh HM, Lee JH, Kim SJ, Chun J. Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int J Syst Evol Microbiol 2005; 55:637–641 [View Article][PubMed]
    [Google Scholar]
  22. Bernardet JF, Bowman JP. Flavobacterium. In William BW. (editor) Bergey's Manual of Systematics of Archaea and Bacteria Hoboken, NJ: John Wiley & Sons, Inc.; 2015 pp. 1–75
    [Google Scholar]
  23. Bernardet JF, Bowman JP. The genus Flavobacterium . In Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E. (editors) The Prokaryotes New York, NY: Springer; 2006 pp. 481–531 [Crossref]
    [Google Scholar]
  24. Goodfellow M, Stackebrandt E. Nucleic Acid Techniques in Bacterial Systematics. Chichester, NY: Wiley; 1991
  25. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  26. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 201633:1870–1874
    [Google Scholar]
  27. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  28. Rzhetsky A, Nei M. A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 1992; 9:945–967
    [Google Scholar]
  29. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  30. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  31. Buck JD. Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 1982; 44:992–993[PubMed]
    [Google Scholar]
  32. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  33. Komagata K, Suzuki KI. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1988; 19:161–207 [Crossref]
    [Google Scholar]
  34. De Ley J. Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 1970; 101:738–754[PubMed]
    [Google Scholar]
  35. Zhu L, Liu Q, Liu H, Zhang J, Dong X et al. Flavobacterium noncentrifugens sp. nov., a psychrotolerant bacterium isolated from glacier meltwater. Int J Syst Evol Microbiol 2013; 63:2032–2037 [View Article][PubMed]
    [Google Scholar]
  36. Li A, Liu H, Sun B, Zhou Y, Xin Y. Flavobacterium lacus sp. nov., isolated from a high-altitude lake, and emended description of Flavobacterium filum . Int J Syst Evol Microbiol 2014; 64:933–939 [View Article][PubMed]
    [Google Scholar]
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