1887

Abstract

Chicory (Cichorium intybus) is a perennial plant (Asteraceae) that grows wild in pasture fields in Saudi Arabia. Chicory plants displaying symptoms typically induced by phytoplasmas, such as bushy phenotype and stunt, were observed in the Mulayda region, Qassim governorate, Saudi Arabia. In this study we examined samples taken from three symptomatic chicory plants and confirmed the presence of phytoplasma DNA. Analysis of the 16S rRNA-encoding sequences showed that the plants were infected with a phytoplasma from the pigeon pea witches’-broom group (16SrIX). Sequencing of the 16S rRNA-encoding gene and the partial cpn60 sequence, computer-simulated RFLP analysis, and phylogenetic analysis of both markers revealed that the phytoplasma identified was representative of a new 16SrIX-J and cpn60 UT IX-IJ subgroup. The present study identified chicory plants as a novel host for phytoplasma strains within the pigeon pea witches’-broom phytoplasma group, and expanded the known diversity of this group.

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2018-01-05
2019-10-20
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References

  1. Lee IM, Davis RE, Gundersen-Rindal DE. Phytoplasma: phytopathogenic mollicutes. Annu Rev Microbiol 2000; 54: 221– 255 [CrossRef] [PubMed]
    [Google Scholar]
  2. Maejima K, Oshima K, Namba S. Exploring the phytoplasmas, plant pathogenic bacteria. J Gen Plant Pathol 2014; 80: 210– 221 [CrossRef]
    [Google Scholar]
  3. Wei W, Davis RE, Lee IM, Zhao Y. Computer-simulated RFLP analysis of 16S rRNA genes: identification of ten new phytoplasma groups. Int J Syst Evol Microbiol 2007; 57: 1855– 1867 [CrossRef] [PubMed]
    [Google Scholar]
  4. Lee IM, Gundersen-Rindal DE, Davis RE, Bartoszyk IM. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int J Syst Bacteriol 1998; 48: 1153– 1169 [CrossRef]
    [Google Scholar]
  5. Zhao Y, Wei W, Lee IM, Shao J, Suo X et al. Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). Int J Syst Evol Microbiol 2009; 59: 2582– 2593 [CrossRef] [PubMed]
    [Google Scholar]
  6. Pérez-López E, Dumonceaux TJ. Detection and identification of the heterogeneous novel subgroup 16SrXIII-(A/I)I phytoplasma associated with strawberry green petal disease and Mexican periwinkle virescence. Int J Syst Evol Microbiol 2016; 66: 4406– 4415 [CrossRef] [PubMed]
    [Google Scholar]
  7. Dumonceaux TJ, Green M, Hammond C, Perez E, Olivier C. Molecular diagnostic tools for detection and differentiation of phytoplasmas based on chaperonin-60 reveal differences in host plant infection patterns. PLoS One 2014; 9: e116039 [CrossRef] [PubMed]
    [Google Scholar]
  8. Fernández FD, Galdeano E, Kornowski MV, Arneodo JD, Conci LR. Description of 'Candidatus Phytoplasma meliae', a phytoplasma associated with Chinaberry (Melia azedarach L.) yellowing in South America. Int J Syst Evol Microbiol 2016; 66: 5244– 5251 [CrossRef] [PubMed]
    [Google Scholar]
  9. Goh SH, Potter S, Wood JO, Hemmingsen SM, Reynolds RP et al. HSP60 gene sequences as universal targets for microbial species identification: studies with coagulase-negative staphylococci. J Clin Microbiol 1996; 34: 818– 823 [PubMed]
    [Google Scholar]
  10. Pérez-López E, Olivier CY, Luna-Rodríguez M, Dumonceaux TJ. Phytoplasma classification and phylogeny based on in silico and in vitro RFLP analysis of cpn60 universal target sequences. Int J Syst Evol Microbiol 2016; 66: 5600– 5613 [CrossRef] [PubMed]
    [Google Scholar]
  11. Alloush GA, Belesky DP, Clapham WM. Forage chicory: a plant resource for nutrient-rich sites. J Agron Crop Sci 2003; 189: 96– 104 [CrossRef]
    [Google Scholar]
  12. Street RA, Sidana J, Prinsloo G. Cichorium intybus: traditional uses, phytochemistry, pharmacology, and toxicology. Evid Based Complement Alternat Med 2013; 2013: 1– 13 [CrossRef] [PubMed]
    [Google Scholar]
  13. Tran-Nguyen LTT, Persley DM, Gibb KS. First report of phytoplasma disease in capsicum, celery and chicory in Queensland, Australia. Australas Plant Pathol 2003; 32: 559– 560 [CrossRef]
    [Google Scholar]
  14. Li ZN, Zhang L, Bai YB, Liu P, Wu YF. Detection and identification of the elm yellows group phytoplasma associated with Puna chicory flat stem in China. Can J Plant Pathol 2012; 34: 34– 41 [Crossref]
    [Google Scholar]
  15. Alfaro-Fernández A, del Cebrián MC, Villaescusa FJ, Font-San-Ambrosio MI. Detection and identification of aster yellows and stolbur phytoplasmas in various crops in Spain. Bull Insectol, Article 2011; 64: S63– S64
    [Google Scholar]
  16. Pérez-López E, Olivier CY, Luna-Rodríguez M, Rodríguez Y, Iglesias LG et al. Maize bushy stunt phytoplasma affects native corn at high elevations in Southeast Mexico. Eur J Plant Pathol 2016; 145: 963– 971 [CrossRef]
    [Google Scholar]
  17. Gundersen DE, Lee IM. Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathol Mediterr 1996; 35: 144– 151
    [Google Scholar]
  18. Olivier CY, Galka B, Séguin-Swartz G. Detection of aster yellows phytoplasma DNA in seed and seedlings of canola (Brassica napus and B. rapa) and AY strain identification. Can J Plant Pathol 2010; 32: 298– 305 [CrossRef]
    [Google Scholar]
  19. Verdin E, Salar P, Danet JL, Choueiri E, Jreijiri F et al. 'Candidatus phytoplasma phoenicium' sp. nov., a novel phytoplasma associated with an emerging lethal disease of almond trees in Lebanon and Iran. Int J Syst Evol Microbiol 2003; 53: 833– 838 [CrossRef] [PubMed]
    [Google Scholar]
  20. Quaglino F, Kube M, Jawhari M, Abou-Jawdah Y, Siewert C et al. 'Candidatus Phytoplasma phoenicium' associated with almond witches'-broom disease: from draft genome to genetic diversity among strain populations. BMC Microbiol 2015; 15: 148 [CrossRef] [PubMed]
    [Google Scholar]
  21. Pérez-López E, Luna-Rodríguez M, Olivier CY, Dumonceaux TJ. The underestimated diversity of phytoplasmas in Latin America. Int J Syst Evol Microbiol 2016; 66: 492– 513 [CrossRef] [PubMed]
    [Google Scholar]
  22. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30: 2725– 2729 [CrossRef] [PubMed]
    [Google Scholar]
  23. Khan AJ, Al-Subhi AM, Calari A, Al-Saady NA, Bertaccini A. A new phytoplasma associated with witches' broom of Cassia italica in Oman. Bull Insectol 2007; 60: 269– 270
    [Google Scholar]
  24. Esmailzadeh Hosseini SA, Babaee G, Salehi M, Mirchenari SM, Bertaccini A. First report of a 'Candidatus Phytoplasma phoenicium'-related strain (16SrIX-I) associated with yellowing of Onobrychis viciifolia in Iran. New Disease Reports 2016; 34: 30 [CrossRef]
    [Google Scholar]
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