1887

Abstract

An obligately aerobic extremely halophilic alkalithermophilic archaeon, strain JW/NM-HA 15, was isolated from the sediments of Wadi An Natrun in Egypt. Phylogenetic analysis based on 16S rRNA and gene sequences indicated that it belongs to the family of the order . The closest relatives were IHC-005 and N-1311 (95.3 and 94.5 % 16S rRNA gene sequence similarity, respectively). Genome relatedness between strain JW/NM-HA 15 and its neighbours was evaluated using average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity with the values of 75.7–85.0, 18.1–20.0, and 70.2–71.0%, respectively. Cells were obligately aerobic, rod-shaped, non-motile, Gram-stain-negative and chemo-organotrophic. The strain grew in the presence of 2.57 M to saturating Na (optimum 3.25–4.60 M Na), at pH 7.5–10.5 (optimum pH 9.0–9.5), and at 30–56 °C (optimum 52 °C). The major polar lipids consisted of phosphatidylglycerol, methylated phosphatidylglycerolphosphate and two phospholipids. The complete genome size of strain JW/NM-HA 15 is approximately 3.93 Mb, with a DNA G+C content of 64.1 mol%. On the basis of phylogenetic features, genomic relatedness, phenotypic and chemotaxonomic data, strain JW/NM-HA 15 was thus considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is JW/NM-HA 15 (=ATCC BAA-2088 =DSM 23470).

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2018-02-01
2019-12-15
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