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Volume 68, Issue 1

sp. nov., a marine alphaproteobacterium, reclassification of as comb. nov. and emended description of the genus Free

Abstract

A Gram-stain-negative, rod-shaped bacterium, designated 157, was isolated from seawater collected from the Philippine Sea. Cells of strain 157 grew in medium containing 0.5–10.0 % NaCl (w/v, optimum 3 %), at pH 6.0–8.5 (optimum 7.0) and at 15–40 °C (optimum 30 °C). Tweens 20, 40 and 80 as well as urea were hydrolysed. The 16S rRNA gene sequence of strain 157 had a high sequence similarity with respect to AZO-C (97.2 %), and exhibited less than 97.0 % sequence similarity to other type strains of the species with validly published names. Phylogenetic analyses revealed that strain 157 fell within a cluster comprising the species and formed a coherent clade with AZO-C and Ar-45. Strain 157 exhibited average nucleotide identity values of 74.5 and 74.9 % to LMG 23705 and Ar-45, respectively. DNA–DNA hybridization analysis revealed that strain 157 shared 20.2 % DNA relatedness with LMG 23705 and 20.6 % with Ar-45, respectively. The sole isoprenoid quinone was ubiquinone 10. The major fatty acids were summed feature 8 (Cω7 and/or Cω6), C cyclo 8, C 2-OH and C. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content was 64.6 mol%. According to the phylogenetic, chemotaxonomic and phenotypic data, it represents a novel species of the genus , for which the name is proposed. The type strain is 157 (=KCTC 52654=MCCC 1K03317). In addition, the description of the genus is emended and is reclassified as comb. nov., with the type strain DY470 (=CGMCC 1.12664=LMG 27871) proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Alphaproteobacteria , phylogenetic analysis , Pseudooceanicola , Rhodobacteraceae and whole genome sequencing
© 2018 IUMS
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2018-01-01
2024-04-24
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Pseudooceanicola lipolyticus sp. nov., a marine alphaproteobacterium, reclassification of Oceanicola flagellatus as Pseudooceanicola flagellatus comb. nov. and emended description of the genus Pseudooceanicola
Int J Syst Evol Microbiol 68, 409 (2018); https://doi.org/10.1099/ijsem.0.002521
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