1887

Abstract

A Gram-stain-negative, rod-shaped bacterium, designated 157, was isolated from seawater collected from the Philippine Sea. Cells of strain 157 grew in medium containing 0.5–10.0 % NaCl (w/v, optimum 3 %), at pH 6.0–8.5 (optimum 7.0) and at 15–40 °C (optimum 30 °C). Tweens 20, 40 and 80 as well as urea were hydrolysed. The 16S rRNA gene sequence of strain 157 had a high sequence similarity with respect to Pseudooceanicola marinus AZO-C (97.2 %), and exhibited less than 97.0 % sequence similarity to other type strains of the species with validly published names. Phylogenetic analyses revealed that strain 157 fell within a cluster comprising the Pseudooceanicola species and formed a coherent clade with P. marinus AZO-C and Pseudooceanicola antarcticus Ar-45. Strain 157 exhibited average nucleotide identity values of 74.5 and 74.9 % to P. marinus LMG 23705 and P. antarcticus Ar-45, respectively. In silico DNA–DNA hybridization analysis revealed that strain 157 shared 20.2 % DNA relatedness with P. marinus LMG 23705 and 20.6 % with P. antarcticus Ar-45, respectively. The sole isoprenoid quinone was ubiquinone 10. The major fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C19 : 0 cyclo ω8c, C16 : 0 2-OH and C16 : 0. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content was 64.6 mol%. According to the phylogenetic, chemotaxonomic and phenotypic data, it represents a novel species of the genus Pseudooceanicola , for which the name Pseudooceanicola lipolyticus is proposed. The type strain is 157 (=KCTC 52654=MCCC 1K03317). In addition, the description of the genus Pseudooceanicola is emended and Oceanicola flagellatus is reclassified as Pseudooceanicola flagellatus comb. nov., with the type strain DY470 (=CGMCC 1.12664=LMG 27871) proposed.

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2017-12-08
2019-10-22
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