1887

Abstract

A Gram-stain-negative, rod-shaped isolate, MBT G8648, was obtained from an acid curd cheese called Quargel. The isolate was moderately salt tolerant and motile, with numerous peritrichous flagella. The 16S rRNA gene sequence analysis indicated that the strain belongs to the genus , with 98.42 % 16S rRNA gene sequence similarity with BH1 as nearest related neighbour. Further comparative sequence analysis of and genes, as well as physiological and biochemical tests, revealed that this bacterium formed a taxon well-separated from its nearest neighbours and other established species. Thus, the strain represents a new species, for which the name sp. nov. is proposed, with strain MBT G8648 (=LMG 29097 =DSM 105749) as type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002515
2018-01-01
2019-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/1/371.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002515&mimeType=html&fmt=ahah

References

  1. Vreeland RH, Litchfield CD, Martin EL, Elliot E. Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int J Syst Bacteriol 1980;30:485–495 [CrossRef]
    [Google Scholar]
  2. Jiang W, Li C, Xu B, Dong X, Ma N et al. Halomonas shantousis sp. nov., a novel biogenic amines degrading bacterium isolated from Chinese fermented fish sauce. Antonie van Leeuwenhoek 2014;106:1073–1080 [CrossRef][PubMed]
    [Google Scholar]
  3. Poli A, Nicolaus B, Denizci AA, Yavuzturk B, Kazan D. Halomonas smyrnensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 2013;63:10–18 [CrossRef][PubMed]
    [Google Scholar]
  4. Yoon JH, Lee KC, Kho YH, Kang KH, Kim CJ et al. Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 2002;52:123–130 [CrossRef][PubMed]
    [Google Scholar]
  5. Jeong SH, Lee JH, Jung JY, Lee SH, Park MS et al. Halomonas cibimaris sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Antonie van Leeuwenhoek 2013;103:503–512 [CrossRef][PubMed]
    [Google Scholar]
  6. Kim MS, Roh SW, Bae JW. Halomonas jeotgali sp. nov., a new moderate halophilic bacterium isolated from a traditional fermented seafood. J Microbiol 2010;48:404–410 [CrossRef][PubMed]
    [Google Scholar]
  7. Amouric A, Liebgott PP, Joseph M, Brochier-Armanet C, Lorquin J. Halomonas olivaria sp. nov., a moderately halophilic bacterium isolated from olive-processing effluents. Int J Syst Evol Microbiol 2014;64:46–54 [CrossRef][PubMed]
    [Google Scholar]
  8. Sørensen MC, Gencay YE, Birk T, Baldvinsson SB, Jäckel C et al. Primary isolation strain determines both phage type and receptors recognised by Campylobacter jejuni bacteriophages. PLoS One 2015;10:e0116287 [CrossRef][PubMed]
    [Google Scholar]
  9. Kostinek M, Specht I, Edward VA, Schillinger U, Hertel C et al. Diversity and technological properties of predominant lactic acid bacteria from fermented cassava used for the preparation of Gari, a traditional African food. Syst Appl Microbiol 2005;28:527–540 [CrossRef][PubMed]
    [Google Scholar]
  10. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  11. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012;19:455–477 [CrossRef][PubMed]
    [Google Scholar]
  12. Aziz RK, Bartels D, Best AA, De Jongh M, Disz T et al. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 2008;9:75 [CrossRef][PubMed]
    [Google Scholar]
  13. Overbeek R, Olson R, Pusch GD, Olsen GJ, Davis JJ et al. The SEED and the rapid annotation of microbial genomes using subsystems technology (RAST). Nucleic Acids Res 2014;42:D206–D214 [CrossRef][PubMed]
    [Google Scholar]
  14. Romano I, Lama L, Nicolaus B, Poli A, Gambacorta A et al. Halomonas alkaliphila sp. nov., a novel halotolerant alkaliphilic bacterium isolated from a salt pool in Campania (Italy). J Gen Appl Microbiol 2006;52:339–348 [CrossRef][PubMed]
    [Google Scholar]
  15. Sánchez-Porro C, Kaur B, Mann H, Ventosa A. Halomonas titanicae sp. nov., a halophilic bacterium isolated from the RMS Titanic. Int J Syst Evol Microbiol 2010;60:2768–2774 [CrossRef][PubMed]
    [Google Scholar]
  16. Koh HW, Rani S, Kim SJ, Moon E, Nam SW et al. Halomonas aestuarii sp. nov., a moderately halophilic bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 2017;67:4298–4303 [CrossRef][PubMed]
    [Google Scholar]
  17. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14:60 [CrossRef][PubMed]
    [Google Scholar]
  18. de La Haba RR, Márquez MC, Papke RT, Ventosa A. Multilocus sequence analysis of the family Halomonadaceae. Int J Syst Evol Microbiol 2012;62:520–538 [CrossRef][PubMed]
    [Google Scholar]
  19. Kaye JZ, Márquez MC, Ventosa A, Baross JA. Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: halophilic bacteria isolated from deep-sea hydrothermal-vent environments. Int J Syst Evol Microbiol 2004;54:499–511 [CrossRef][PubMed]
    [Google Scholar]
  20. Jiang J, Pan Y, Meng L, Hu S, Zhang X et al. Halomonas zhaodongensis sp. nov., a slightly halophilic bacterium isolated from saline-alkaline soils in Zhaodong, China. Antonie van Leeuwenhoek 2013;104:685–694 [CrossRef][PubMed]
    [Google Scholar]
  21. Long MR, Zhang DF, Yang XY, Zhang XM, Zhang YG et al. Halomonas nanhaiensis sp. nov., a halophilic bacterium isolated from a sediment sample from the South China Sea. Antonie van Leeuwenhoek 2013;103:997–1005 [CrossRef][PubMed]
    [Google Scholar]
  22. Chen YG, Zhang YQ, Huang HY, Klenk HP, Tang SK et al. Halomonas zhanjiangensis sp. nov., a halophilic bacterium isolated from a sea urchin. Int J Syst Evol Microbiol 2009;59:2888–2893 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002515
Loading
/content/journal/ijsem/10.1099/ijsem.0.002515
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error