%0 Journal Article %A Riojas, Marco A. %A McGough, Katya J. %A Rider-Riojas, Cristin J. %A Rastogi, Nalin %A Hazbón, Manzour Hernando %T Phylogenomic analysis of the species of the Mycobacterium tuberculosis complex demonstrates that Mycobacterium africanum, Mycobacterium bovis, Mycobacterium caprae, Mycobacterium microti and Mycobacterium pinnipedii are later heterotypic synonyms of Mycobacterium tuberculosis %D 2018 %J International Journal of Systematic and Evolutionary Microbiology, %V 68 %N 1 %P 324-332 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002507 %K Mycobacterium tuberculosis complex %K Mycobacterium tuberculosis %K average nucleotide identity %K digital DNA–DNA hybridization %K species reclassification %I Microbiology Society, %X The species within the Mycobacterium tuberculosis Complex (MTBC) have undergone numerous taxonomic and nomenclatural changes, leaving the true structure of the MTBC in doubt. We used next-generation sequencing (NGS), digital DNA–DNA hybridization (dDDH), and average nucleotide identity (ANI) to investigate the relationship between these species. The type strains of Mycobacterium africanum , Mycobacterium bovis , Mycobacterium caprae , Mycobacterium microti and Mycobacterium pinnipedii were sequenced via NGS. Pairwise dDDH and ANI comparisons between these, previously sequenced MTBC type strain genomes (including ‘Mycobacterium canettii’, ‘Mycobacterium mungi’ and ‘Mycobacterium orygis’) and M. tuberculosis H37RvT were performed. Further, all available genome sequences in GenBank for species in or putatively in the MTBC were compared to H37RvT. Pairwise results indicated that all of the type strains of the species are extremely closely related to each other (dDDH: 91.2–99.2 %, ANI: 99.21–99.92 %), greatly exceeding the respective species delineation thresholds, thus indicating that they belong to the same species. Results from the GenBank genomes indicate that all the strains examined are within the circumscription of H37RvT (dDDH: 83.5–100 %). We, therefore, formally propose a union of the species of the MTBC as M. tuberculosis . M. africanum , M. bovis , M. caprae , M. microti and M. pinnipedii are reclassified as later heterotypic synonyms of M. tuberculosis . ‘M. canettii’, ‘M. mungi’, and ‘M. orygis’ are classified as strains of the species M. tuberculosis . We further recommend use of the infrasubspecific term ‘variant’ (‘var.’) and infrasubspecific designations that generally retain the historical nomenclature associated with the groups or otherwise convey such characteristics, e.g. M. tuberculosis var. bovis. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002507