1887

Abstract

We conducted a taxonomic study of two algicidal bacteria, designated strains Yeonmyeong 1-13 and Yeonmyeong 1-11, isolated from seawater off Geoje Island in the South Sea, Republic of Korea. The two novel strains were yellow-pigmented, halotolerant, Gram-stain-negative, strictly aerobic, non-spore-forming, rod-shaped bacteria. Both strains were able to grow at 5–39 °C, pH 5.0–10.0 and 0–11 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strains Yeonmyeong 1-13 and Yeonmyeong 1-11 belonged to the genus Stakelama and are closely related to Stakelama pacifica JLT832 (98.37% and 98.22 % sequence similarity, respectively). The pairwise sequence similarity between strains Yeonmyeong 1-13 and Yeonmyeong 1-11 was observed to be 99.50 %. In both strains, the only respiratory quinone was ubiquinone-10; the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid; the major fatty acids were C18 : 1ω7c, C16 : 0 and C14 : 0 2-OH. DNA G+C content values of strains Yeonmyeong 1-13 and Yeonmyeong 1-11 were 65.1% and 64.9 mol%, respectively. The DNA–DNA relatedness between Yeonmyeong 1-13 and S. pacifica DSM 25059 was 28.7 %, which falls below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished strain Yeonmyeong 1-13 from its closest phylogenetic neighbours. Thus, strains Yeonmyeong 1-13 and Yeonmyeong 1-11 represent a novel species of the genus Stakelama , for which the name Stakelama algicida sp. nov. is proposed. The type strain is Yeonmyeong 1-13 (=KEMB 9005-324=JCM 31498).

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2017-12-05
2019-12-08
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