%0 Journal Article %A Lee, Yunho %A Jeon, Che Ok %T Methylobacterium frigidaeris sp. nov., isolated from an air conditioning system %D 2018 %J International Journal of Systematic and Evolutionary Microbiology, %V 68 %N 1 %P 299-304 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002500 %K air-conditioning system %K Alphaproteobacteria %K Methylobacterium frigidaeris %K new taxa %I Microbiology Society, %X A reddish pink-pigmented, Gram-stain-negative, aerobic and methylotrophic bacterial strain, designated strain IER25-16T, was isolated from a laboratory air conditioning system in the Republic of Korea. Cells were motile rods showing catalase- and oxidase-positive reactions. Strain IER25-16T grew at 10–40 °C (optimum, 30 °C), at pH 4.0–7.0 (optimum, pH 5.0–7.0) and in the presence of 0–1.0 % (w/v) NaCl (optimum, 0 %). The major respiratory quinone was ubiquinone-10 and ubiquinone-9 was also detected as the minor respiratory quinone. Summed feature 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c) was detected as the predominant fatty acids. The genomic DNA G+C content of strain IER25-16T was 70.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain IER25-16T belonged to the genus Methylobacterium of the class Alphaproteobacteria . Strain IER25-16T was most closely related to Methylobacterium platani PMB02T (97.9 %), Methylobacterium aquaticum GR16T (97.9 %) and Methylobacterium tarhaniae N4211T (97.5 %). The average nucleotide identity and in silico DNA–DNA hybridization values between strain IER25-16T and M. platani , M. aquaticum and M. tarhaniae were 88.3, 88.8 and 89.6 % and 36.2, 37.3 and 39.3 %, respectively. The phenotypic and chemotaxonomic features and the phylogenetic inference clearly suggested that strain IER25-16T represents a novel species of the genus Methylobacterium , for which the name Methylobacterium frigidaeris sp. nov. is proposed. The type strain is strain IER25-16T (=KACC 19280T=JCM 32048T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002500