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Abstract

A bacterial strain designated TP462, isolated from a seamount near the Yap Trench in the tropical western Pacific, was characterized using a polyphasic taxonomic approach. Strain TP462 was found to be Gram-stain-negative, aerobic, rod-shaped and motile by means of a single polar flagellum. Growth occurred at 4–37 °C (optimum, 25–30 °C) and with 0–4.0 % NaCl (optimum, 2–3 %). Phylogenetic analysis based on 16S rRNA gene sequence showed that strain TP462 was related to the genus Rheinheimera and had the highest 16S rRNA gene sequence similarity with the type strain Rheinheimera tangshanensis JA3-B52 (96.8 %). The predominant cellular fatty acids were C17 : 1ω8c, summed feature 3 (composed of iso-C15 : 0 2-OH and/or C16 : 1ω7c) and C16 : 0. The polar lipid profile contained phosphatidylglycerol, phosphatidylethanolamine and two unidentified lipids. The genomic DNA G+C content of strain TP462 was 48.7 mol%. On the basis of the evidence presented in this study, strain TP462 represents a novel species of the genus Rheinheimera , for which we propose the name Rheinheimera marina sp. nov. (type strain TP462=KACC 18560=CGMCC 1.15399).

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2017-11-21
2019-12-08
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References

  1. Brettar I, Christen R, Höfle MG. Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic Sea. Int J Syst Evol Microbiol 2002;52:1851–1857 [CrossRef][PubMed]
    [Google Scholar]
  2. Romanenko LA, Uchino M, Falsen E, Zhukova NV, Mikhailov VV et al. Rheinheimera pacifica sp. nov., a novel halotolerant bacterium isolated from deep sea water of the Pacific. Int J Syst Evol Microbiol 2003;53:1973–1977 [CrossRef][PubMed]
    [Google Scholar]
  3. Yoon JH, Bae SE, Park SE, Kang SJ, Oh TK. Rheinheimera aquimaris sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 2007;57:1386–1390 [CrossRef][PubMed]
    [Google Scholar]
  4. Li HJ, Zhang XY, Zhang YJ, Zhou MY, Gao ZM et al. Rheinheimera nanhaiensis sp. nov., isolated from marine sediments, and emended description of the genus Rheinheimera Brettar et al. 2002 emend. Merchant et al. 2007. Int J Syst Evol Microbiol 2011;61:1016–1022 [CrossRef][PubMed]
    [Google Scholar]
  5. Park S, Park JM, Won SM, Jung YT, Yoon JH. Rheinheimera arenilitoris sp. nov., isolated from seashore sand. Int J Syst Evol Microbiol 2014;64:3749–3754 [CrossRef][PubMed]
    [Google Scholar]
  6. Baek K, Jeon CO. Rheinheimera aestuari sp. nov., a marine bacterium isolated from coastal sediment. Int J Syst Evol Microbiol 2015;65:2640–2645 [CrossRef][PubMed]
    [Google Scholar]
  7. Romanenko LA, Tanaka N, Svetashev VI, Kalinovskaya NI, Mikhailov VV. Rheinheimera japonica sp. nov., a novel bacterium with antimicrobial activity from seashore sediments of the Sea of Japan. Arch Microbiol 2015;197:613–620 [CrossRef][PubMed]
    [Google Scholar]
  8. Zhang DC, Liu YX, Huang HJ, Wu J. Pseudoalteromonas profundi sp. nov., isolated from a deep-sea seamount. Int J Syst Evol Microbiol 2016;66:4416–4421 [CrossRef][PubMed]
    [Google Scholar]
  9. Zhang DC, Liu YX, Huang HJ. Novosphingobium profundi sp. nov. isolated from a deep-sea seamount. Antonie van Leeuwenhoek 2017;110:19–25 [CrossRef][PubMed]
    [Google Scholar]
  10. Liu J, Sun YW, Zhang DD, Li SN, Zhang DC. Oceanisphaera marina sp. nov., isolated from a deep-sea seamount. Int J Syst Evol Microbiol 2017;67:1996–2000 [CrossRef][PubMed]
    [Google Scholar]
  11. Sambrook J, Russell DW. (editors) Molecular Cloning: a Laboratory Manual Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press; 2001
    [Google Scholar]
  12. Zhang DC, Redzic M, Schinner F, Margesin R. Glaciimonas immobilis gen. nov., sp. nov., a member of the family Oxalobacteraceae isolated from alpine glacier cryoconite. Int J Syst Evol Microbiol 2011;61:2186–2190 [CrossRef][PubMed]
    [Google Scholar]
  13. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  14. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  15. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  16. Süßmuth R, Eberspächer J, Haag R, Springer W. Biochemisch-Mikrobiologisches Praktikum Stuttgart: Georg Thieme Verlag; 1987
    [Google Scholar]
  17. Dong XZ, Cai MY. (editors) Determinative Manual for Routine Bacteriology Beijing: Scientific Press; 2001
    [Google Scholar]
  18. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, Technical Note 101. Newark, DE: MIDI; 1990
    [Google Scholar]
  19. Zhang X, Sun L, Qiu F, McLean RJ, Jiang R et al. Rheinheimera tangshanensis sp. nov., a rice root-associated bacterium. Int J Syst Evol Microbiol 2008;58:2420–2424 [CrossRef][PubMed]
    [Google Scholar]
  20. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959;37:911–917 [CrossRef][PubMed]
    [Google Scholar]
  21. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990;66:199–202 [CrossRef]
    [Google Scholar]
  22. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990;13:128–130 [CrossRef]
    [Google Scholar]
  23. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962;5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  24. Brettar I, Christen R, Höfle MG. Rheinheimera perlucida sp. nov., a marine bacterium of the Gammaproteobacteria isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 2006;56:2177–2183 [CrossRef][PubMed]
    [Google Scholar]
  25. de Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970;12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  26. Huss VA, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983;4:184–192 [CrossRef][PubMed]
    [Google Scholar]
  27. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
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