1887

Abstract

A novel Gram-stain-negative, straight or slightly curved rod-shaped, non-spore-forming, non-flagellated, strictly aerobic strain, designated RZG4-3-1, was isolated from coastal seawater of Rizhao, China (119.625° E 35.517° N). The organism grew optimally at 24–28 °C, at pH 7.0 and in the presence of 2.0 % (w/v) NaCl. The strain required seawater or artificial seawater for growth, and NaCl alone did not support growth. Strain RZG4-3-1 contained ubiquinone 8 (Q-8) as the major respiratory quinone and contained C16 : 1 ω7c and/or C16 : 1 ω6c and C16 : 0 as the dominant fatty acids. The polar lipids of strain RZG4-3-1 were phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminophospholipid. The DNA G+C content of strain RZG4-3-1 was 40.1 mol%. Strain RZG4-3-1 exhibited the highest 16S rRNA gene sequence similarity value (96.0 %) to Thalassotalea eurytherma JCM 18482. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RZG4-3-1 belonged to the genus Thalassotalea . On the basis of polyphasic analyses, strain RZG4-3-1 represents a novel species of the genus Thalassotalea , for which the name Thalassotalea atypica sp. nov. is proposed. The type strain is RZG4-3-1 (=JCM 31894=KCTC 52745=MCCC 1K03276). An emended description of Thalassotalea eurytherma is also provided.

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2017-11-16
2019-09-15
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References

  1. Zhang Y, Tang K, Shi X, Zhang XH. Description of Thalassotalea piscium gen. nov., sp. nov., isolated from flounder (Paralichthys olivaceus), reclassification of four species of the genus Thalassomonas as members of the genus Thalassotalea gen. nov. and emended description of the genus Thalassomonas. Int J Syst Evol Microbiol 2014; 64: 1223– 1228 [CrossRef] [PubMed]
    [Google Scholar]
  2. Park S, Choi WC, Oh TK, Yoon JH. Thalassomonas agariperforans sp. nov., an agarolytic bacterium isolated from marine sand. Int J Syst Evol Microbiol 2011; 61: 2573– 2576 [CrossRef] [PubMed]
    [Google Scholar]
  3. Jean WD, Shieh WY, Liu TY. Thalassomonas agarivorans sp. nov., a marine agarolytic bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the genus Thalassomonas. Int J Syst Evol Microbiol 2006; 56: 1245– 1250 [CrossRef] [PubMed]
    [Google Scholar]
  4. Thompson FL, Barash Y, Sawabe T, Sharon G, Swings J et al. Thalassomonas loyana sp. nov., a causative agent of the white plague-like disease of corals on the Eilat coral reef. Int J Syst Evol Microbiol 2006; 56: 365– 368 [CrossRef] [PubMed]
    [Google Scholar]
  5. Yi H, Bae KS, Chun J. Thalassomonas ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 2004; 54: 377– 380 [CrossRef] [PubMed]
    [Google Scholar]
  6. Jung YT, Park S, Yoon JH. Thalassomonas fusca sp. nov., a novel gammaproteobacterium isolated from tidal flat sediment. Antonie van Leeuwenhoek 2014; 105: 81– 87 [CrossRef] [PubMed]
    [Google Scholar]
  7. Sun C, Huo YY, Liu JJ, Pan J, Qi YZ et al. Thalassomonas eurytherma sp. nov., a marine proteobacterium. Int J Syst Evol Microbiol 2014; 64: 2079– 2083 [CrossRef] [PubMed]
    [Google Scholar]
  8. Park S, Jung YT, Kang CH, Park JM, Yoon JH. Thalassotalea ponticola sp. nov., isolated from seawater, reclassification of Thalassomonas fusca as Thalassotalea fusca comb. nov. and emended description of the genus Thalassotalea. Int J Syst Evol Microbiol 2014; 64: 3676– 3682 [CrossRef] [PubMed]
    [Google Scholar]
  9. Hou TT, Liu Y, Zhong ZP, Liu HC, Liu ZP. Thalassotalea marina sp. nov., isolated from a marine recirculating aquaculture system, reclassification of Thalassomonas eurytherma as Thalassotalea eurytherma comb. nov. and emended description of the genus Thalassotalea. Int J Syst Evol Microbiol 2015; 65: 4710– 4715 [CrossRef] [PubMed]
    [Google Scholar]
  10. Chen WM, Liu LP, Chen CA, Wang JT, Sheu SY. Thalassotalea montiporae sp. nov., isolated from the encrusting pore coral Montipora aequituberculata. Int J Syst Evol Microbiol 2016; 66: 4077– 4084 [CrossRef] [PubMed]
    [Google Scholar]
  11. Sheu SY, Liu LP, Tang SL, Chen WM. Thalassotalea euphylliae sp. nov., isolated from the torch coral Euphyllia glabrescens. Int J Syst Evol Microbiol 2016; 66: 5039– 5045 [CrossRef] [PubMed]
    [Google Scholar]
  12. Wang Y, Zhou C, Ming H, Kang J, Chen H et al. Pseudofulvibacter marinus sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2016; 66: 1301– 1305 [CrossRef] [PubMed]
    [Google Scholar]
  13. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613– 1617 [CrossRef] [PubMed]
    [Google Scholar]
  14. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25: 4876– 4882 [CrossRef] [PubMed]
    [Google Scholar]
  15. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16: 111– 120 [CrossRef] [PubMed]
    [Google Scholar]
  16. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33: 1870– 1874 [CrossRef] [PubMed]
    [Google Scholar]
  17. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37: 463– 464 [Crossref]
    [Google Scholar]
  18. Beveridge TJ, Lawrence JR, Murray RGE. Sampling and staining for light microscopy. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology Washington, DC: ASM Press; 2007; pp. 19– 33
    [Google Scholar]
  19. Lyman J, Fleming RH. Composition of seawater. J Mar Res 1940; 3: 134– 146
    [Google Scholar]
  20. Breznak JA, Costilow RN. Physicochemical factors in growth. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: ASM Press; 1994; pp. 137– 154
    [Google Scholar]
  21. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52: 1049– 1070 [CrossRef] [PubMed]
    [Google Scholar]
  22. Tindall BJ, Sikorski J, Smibert RM, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology Washington, DC: ASM Press; 2007; pp. 330– 393
    [Google Scholar]
  23. Hsu SC, Lockwood JL. Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 1975; 29: 422– 426 [PubMed]
    [Google Scholar]
  24. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  25. Collins MD. Isoprenoid quinones. In Goodfellow M, O’Donnell AG. (editors) Chemical Methods in Prokaryotic Systematics Chichester: Wiley Press; 1994; pp. 265– 309
    [Google Scholar]
  26. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
  27. Mesbah M, Whitman WB. Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 1989; 479: 297– 306 [CrossRef] [PubMed]
    [Google Scholar]
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