1887

Abstract

A Gram-stain-negative, strictly aerobic and halotolerant bacterial strain, designated strain NAP41, was isolated from a sea tidal flat in the Yellow Sea of South Korea. Cells were non-motile cocci showing oxidase- and catalase-positive activities. Growth of strain NAP41 was observed at 15–40 °C (optimum, 37 °C), at pH 6.5–9.0 (optimum, pH 7.0–7.5) and in the presence of 0.5–12 % (w/v) NaCl (optimum, 2 %). Strain NAP41 contained summed feature 8 (comprising C 7/ 6) and C as the major fatty acids and ubiquinone-10 as the sole isoprenoid quinone. Phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified aminolipid and three unidentified lipids were detected as the polar lipids. The G+C content of the genomic DNA was 56.0 mol%. Strain NAP41 was most closely related to SSK3-2, YCS-24 and DSM 29430 with 96.67, 96.39 and 96.39 % 16S rRNA gene sequence similarities, respectively, and formed a phylogenetic lineage distinct from closely related taxa within the family with low bootstrap values. On the basis of phenotypic, chemotaxonomic and molecular properties, strain NAP41 representthe name gen. nov., sp. nov. is proposed. The type strain of the type species is NAP41 (KACC 18431=JCM 30739).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002494
2018-01-01
2020-04-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/1/260.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002494&mimeType=html&fmt=ahah

References

  1. Pujalte MJ, Lucena T, Ruvira MA, Arahal DR, Macián MC et al. The family Rhodobacteraceae. In Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F et al. (editors) The Prokaryotes, Alphaproteobacteria and Betaproteobacteria, 4th ed. Berlin: Springer; 2014; pp.545–577
    [Google Scholar]
  2. Buchan A, González JM, Moran MA. Overview of the marine Roseobacter lineage. Appl Environ Microbiol 2005;71:5665–5677 [CrossRef][PubMed]
    [Google Scholar]
  3. Romanenko LA, Tanaka N, Svetashev VI, Mikhailov VV. Primorskyibacter sedentarius gen. nov., sp. nov., a novel member of the class Alphaproteobacteria from shallow marine sediments. Int J Syst Evol Microbiol 2011;61:1572–1578 [CrossRef][PubMed]
    [Google Scholar]
  4. Jin HM, Jeong HI, Jeon CO. Aliiglaciecola aliphaticivorans sp. nov., an aliphatic hydrocarbon-degrading bacterium, isolated from a sea-tidal flat and emended description of the genus Aliiglaciecola Jean et al. 2013. Int J Syst Evol Microbiol 2015;65:1550–1555 [CrossRef][PubMed]
    [Google Scholar]
  5. Baek K, Jeon CO. Rheinheimera gaetbuli sp. nov., a marine bacterium isolated from a tidal flat. Curr Microbiol 2016;72:344–350 [CrossRef][PubMed]
    [Google Scholar]
  6. Kim KH, Jin HM, Jeong HI, Jeon CO. Maribacter lutimaris sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2016;66:1773–1778 [CrossRef][PubMed]
    [Google Scholar]
  7. Jeong HI, Jin HM, Jeon CO. Confluentimicrobium naphthalenivorans sp. nov., a naphthalene-degrading bacterium isolated from sea-tidal-flat sediment, and emended description of the genus Confluentimicrobium Park et al. 2015. Int J Syst Evol Microbiol 2015;65:4191–4195 [CrossRef][PubMed]
    [Google Scholar]
  8. Kim JM, Le NT, Chung BS, Park JH, Bae JW et al. Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. Appl Environ Microbiol 2008;74:7313–7320 [CrossRef][PubMed]
    [Google Scholar]
  9. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  10. Nawrocki EP, Eddy SR. Query-dependent banding (QDB) for faster RNA similarity searches. PLoS Comput Biol 2007;3:e56 [CrossRef][PubMed]
    [Google Scholar]
  11. Felsenstein J. PHYLIP (Phylogeny Inference Package), Version 3.6a Distributed by the author; Department of Genome Sciences, University of Washington, Seattle, USA: 2002
    [Google Scholar]
  12. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014;30:1312–1313 [CrossRef][PubMed]
    [Google Scholar]
  13. Wang Q, Garrity GM, Tiedje JM, Cole JR. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 2007;73:5261–5267 [CrossRef][PubMed]
    [Google Scholar]
  14. Gomori G. Preparation of buffers for use in enzyme studies. Methods Enzymol 1955;1:138–146[Crossref]
    [Google Scholar]
  15. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P. (editor) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  16. Lányi B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987;19:1–67
    [Google Scholar]
  17. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  18. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–208[Crossref]
    [Google Scholar]
  19. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002;4:770–773 [CrossRef][PubMed]
    [Google Scholar]
  20. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  21. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of nocardia and related bacteria. Int J Syst Bacteriol 1977;27:104–117 [CrossRef]
    [Google Scholar]
  22. Park S, Park JM, Jung YT, Won SM, Yoon JH. Primorskyibacter insulae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 2015;65:3971–3976 [CrossRef][PubMed]
    [Google Scholar]
  23. Park S, Jung YT, Kim SI, Yoon JH. Thalassococcus lentus sp. nov., an alphaproteobacterium isolated from seawater of a seaweed farm. Antonie van Leeuwenhoek 2013;103:465–473 [CrossRef][PubMed]
    [Google Scholar]
  24. Park S, Kang SJ, Oh TK, Yoon JH. Roseivivax lentus sp. nov., isolated from a tidal flat sediment, and emended description of the genus Roseivivax Suzuki et al. 1999. Int J Syst Evol Microbiol 2010;60:1113–1117 [CrossRef][PubMed]
    [Google Scholar]
  25. Jung YT, Lee JS, Yoon JH. Maliponia aquimaris gen. nov., sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2016;66:2271–2277 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002494
Loading
/content/journal/ijsem/10.1099/ijsem.0.002494
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error