1887

Abstract

Two novel actinomycete isolates, designated strains NEAU-A4 and NEAU-A3, were isolated from rhizosphere soil of wheat ( L.) and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics of the two strains coincided with those of the genus . The 16S rRNA gene sequence analysis showed that the two isolates exhibited 99.6 % 16S rRNA gene sequence similarity with each other and that they were most closely related to DSM 40279 (98.8, 99.0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains clustered together and formed a separate subclade. Furthermore, a combination of DNA–DNA hybridization results and some physiological and biochemical properties demonstrated that the two strains could be distinguished from its closest relative. Therefore, it is proposed that strains NEAU-A4 and NEAU-A3 should be classified as representatives of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-A4 (=CGMCC 4.7393=DSM 104540).

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2018-02-01
2020-04-09
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