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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 68, Issue 1

sp. nov., isolated from the root and rhizosphere soil of wheat ( L.) Free

Abstract

Two actinomycete strains, NEAU-C11 and NEAU-C8, isolated from rhizosphere soil and wheat root, respectively, collected from Langfang, Hebei Province, China. A polyphasic study was carried out to establish the taxonomic position of the two strains. Morphological and chemotaxonomic characteristics of the isolates coincided with the genus . Sequences analysis of the 16S rRNA gene also showed that the organisms belong to the genus and is the highest sequence match for both strains. Furthermore, a combination of DNA–DNA hybridization results and some differential physiological and biochemical properties indicated that they were distinguishable from the phylogenetically closest relatives. Therefore, the two strains represent a novel species, for which the name sp. nov. is proposed. The type strain is NEAU-C11 (=CGMCC 4.7396=DSM 104646).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Glycomyces rhizosphaerae sp. nov. and polyphasic taxonomy
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2018-01-01
2025-04-21
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References

  1. Labeda DP, Testa RT, Lechevalier MP, Lechevalier HA. Glycomyces, a new genus of the Actinomycetales . Int J Syst Bacteriol 1985; 35:417–421 [View Article]
     [Google Scholar]
  2. Labeda DP, Kroppenstedt RM. Emended description of the genus Glycomyces and description of Glycomyces algeriensis sp. nov., Glycomyces arizonensis sp. nov. and Glycomyces lechevalierae sp. nov. Int J Syst Evol Microbiol 2004; 54:2343–2346 [View Article][PubMed]
     [Google Scholar]
  3. Liu C, Wang X, Zhao J, Liu Q, Wang L et al. Streptomyces harbinensis sp. nov., an endophytic, ikarugamycin-producing actinomycete isolated from soybean root [Glycine max (L.) Merr]. Int J Syst Evol Microbiol 2013; 63:3579–3584 [View Article][PubMed]
     [Google Scholar]
  4. Li YQ, Liu L, Cheng C, Shi XH, Lu CY et al. Saccharothrix lopnurensis sp. nov., a filamentous actinomycete isolated from sediment of Lop Nur. Antonie van Leeuwenhoek 2015; 108:975–981 [View Article][PubMed]
     [Google Scholar]
  5. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966; 16:313–340 [View Article]
     [Google Scholar]
  6. Jones KL. Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 1949; 57:141–145[PubMed]
     [Google Scholar]
  7. Waksman SA. The Actinobacteria . In Classification, Identification and Descriptions of Genera and Species vol. 2 Baltimore: Williams and Wilkins; 1961
     [Google Scholar]
  8. Waksman SA. The Actinomycetes . In A Summary of Current Knowledge New York: Ronald Press; 1967
     [Google Scholar]
  9. Kelly KL. Inter-society color council-national bureau of standards color-name charts illustrated with centroid colors published in US; 1964
  10. Jia F, Liu C, Wang X, Zhao J, Liu Q et al. Wangella harbinensis gen. nov., sp. nov., a new member of the family Micromonosporaceae . Antonie van Leeuwenhoek 2013; 103:399–408 [View Article][PubMed]
     [Google Scholar]
  11. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
     [Google Scholar]
  12. Gordon RE, Barnett DA, Handerhan JE, Pang CH-N. Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 1974; 24:54–63 [View Article]
     [Google Scholar]
  13. Yokota A, Tamura T, Hasegawa T, Huang LH. Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . Int J Syst Bacteriol 1993; 43:805–812 [View Article]
     [Google Scholar]
  14. McKerrow J, Vagg S, McKinney T, Seviour EM, Maszenan AM et al. A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. Lett Appl Microbiol 2000; 30:178–182 [View Article][PubMed]
     [Google Scholar]
  15. Lechevalier MP, Lechevalier HA. The chemotaxonomy of actinomycetes. In Dietz A, Thayer DW. (editors) Actinomycete Taxonomy (Special Publication) vol. 6 Arlington: Society of Industrial Microbiology; 1980 pp. 227–291
     [Google Scholar]
  16. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  17. Collins MD. Isoprenoid quinone analyses in bacterial classification and identification. In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics London: Academic Press; 1985 pp. 267–284
     [Google Scholar]
  18. Wu C, Lu X, Qin M, Wang Y, Ruan J. Analysis of menaquinone compound in microbial cells by HPLC. Microbiology 1989; 16:176–178
     [Google Scholar]
  19. Gao R, Liu C, Zhao J, Jia F, Yu C et al. Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora . Antonie van Leeuwenhoek 2014; 105:307–315 [View Article][PubMed]
     [Google Scholar]
  20. Xiang W, Liu C, Wang X, Du J, Xi L et al. Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). Int J Syst Evol Microbiol 2011; 61:1165–1169 [View Article][PubMed]
     [Google Scholar]
  21. Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M. Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 1980; 188:221–233 [View Article]
     [Google Scholar]
  22. Kim SB, Brown R, Oldfield C, Gilbert SC, Iliarionov S et al. Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. Int J Syst Evol Microbiol 2000; 50:2031–2036 [View Article][PubMed]
     [Google Scholar]
  23. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  24. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
     [Google Scholar]
  25. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  26. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  27. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
     [Google Scholar]
  28. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
     [Google Scholar]
  29. Mandel M, Marmur J. Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 1968; 12:195–206 [Crossref]
     [Google Scholar]
  30. de Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970; 12:133–142 [View Article][PubMed]
     [Google Scholar]
  31. Huss VA, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983; 4:184–192 [View Article][PubMed]
     [Google Scholar]
  32. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [View Article]
     [Google Scholar]
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Glycomyces rhizosphaerae sp. nov., isolated from the root and rhizosphere soil of wheat (Triticum aestivum L.)
Int J Syst Evol Microbiol 68, 223 (2018); https://doi.org/10.1099/ijsem.0.002488
/content/journal/ijsem/10.1099/ijsem.0.002488
/content/journal/ijsem/10.1099/ijsem.0.002488
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