1887

Abstract

A Gram-stain-positive, facultatively anaerobic bacterial strain, GDSW-R2A3, was isolated from a downstream water sample collected from the river Ganges, India. Analysis of the 16S rRNA gene sequence of strain GDSW-R2A3 revealed its affiliation to the family . Further analysis using a polyphasic approach revealed that strain GDSW-R2A3 was most closely related to the genus . Analysis of the almost-complete (1488 bp) 16S rRNA gene sequence of strain GDSW-R2A3 revealed the highest level of sequence similarity with CCM 8426 (98.3 %) and KCTC 13712 (98.3 %) followed by DSM 14730 (98.0 %). The digital DNA–DNA hybridization and average nucleotide identity (ANI) values between strain GDSW-R2A3 and the most closely related taxon, CCM 8426, were 20.3 and 78.2 %, respectively. The DNA G+C content of the strain was 44.2 mol%. The cell-wall amino acid was -diaminopimelic acid. Polar lipids present were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, three aminophospholipids, two phospholipids and one unidentified lipid; the major menaquinone was MK-7; iso-C, iso-C and anteiso-C were the major fatty acids. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, it can be concluded that strain GDSW-R2A3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GDSW-R2A3 (=VTCC-B-910015=CCM 8782).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002474
2018-01-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/1/160.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002474&mimeType=html&fmt=ahah

References

  1. Glaeser SP, Dott W, Busse HJ, Kämpfer P. Fictibacillus phosphorivorans gen. nov., sp. nov. and proposal to reclassify Bacillus arsenicus, Bacillus barbaricus, Bacillus macauensis, Bacillus nanhaiensis, Bacillus rigui, Bacillus solisalsi and Bacillus gelatini in the genus Fictibacillus. Int J Syst Evol Microbiol 2013;63:2934–2944 [CrossRef][PubMed]
    [Google Scholar]
  2. Baik KS, Lim CH, Park SC, Kim EM, Rhee MS et al. Bacillus rigui sp. nov., isolated from wetland fresh water. Int J Syst Evol Microbiol 2010;60:2204–2209 [CrossRef][PubMed]
    [Google Scholar]
  3. Liu H, Zhou Y, Liu R, Zhang KY, Lai R. Bacillus solisalsi sp. nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake. Int J Syst Evol Microbiol 2009;59:1460–1464 [CrossRef][PubMed]
    [Google Scholar]
  4. Dastager SG, Mawlankar R, Srinivasan K, Tang SK, Lee JC et al. Fictibacillus enclensis sp. nov., isolated from marine sediment. Antonie van Leeuwenhoek 2014;105:461–469 [CrossRef][PubMed]
    [Google Scholar]
  5. Sharma A, Kohli P, Singh Y, Schumann P, Lal R. Fictibacillus halophilus sp. nov., from a microbial mat of a hot spring atop the Himalayan Range. Int J Syst Evol Microbiol 2016;66:2409–2416 [CrossRef][PubMed]
    [Google Scholar]
  6. Logan NA, Berge O, Bishop AH, Busse HJ, de Vos P et al. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 2009;59:2114–2121 [CrossRef][PubMed]
    [Google Scholar]
  7. Murray RGE, Doetsch RN, Robinow CF. Determinative and cytological light microscopy. In Gerhard P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.36
    [Google Scholar]
  8. Dhanjal S, Ruckmani A, Cameotra SS, Pukall R, Klenk HP et al. Yaniella fodinae sp. nov., isolated from a coal mine. Int J Syst Evol Microbiol 2011;61:535–539 [CrossRef][PubMed]
    [Google Scholar]
  9. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005;55:1149–1153 [CrossRef][PubMed]
    [Google Scholar]
  10. Cowan ST, Steel KJ. Manual for the Identification of Medical Bacteria London: Cambridge University Press; 1965
    [Google Scholar]
  11. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  12. Lanyi B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987;19:1–67
    [Google Scholar]
  13. Smith NR, Gordon RE, Clark FE. Aerobic Spore Forming Bacteria Washington, DC: Agriculture Monograph No. 16, United States Department of Agriculture; 1952
    [Google Scholar]
  14. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959;37:911–917 [CrossRef][PubMed]
    [Google Scholar]
  15. Komagata K, Suzuki K. Lipid and cell wall analysis in bacterial systematics. In Booth C, Bergan T, Bennett PM, Brown AJP, Colwell RR et al. (editors) Methods in Microbiology St. Louis, MO, USA: Academic Press; 1987; pp.99
    [Google Scholar]
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  17. Pandey KK, Mayilraj S, Chakrabarti T. Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 2002;52:1559–1567 [CrossRef][PubMed]
    [Google Scholar]
  18. Mayilraj S, Saha P, Suresh K, Saini HS. Ornithinimicrobium kibberense sp. nov., isolated from the Indian Himalayas. Int J Syst Evol Microbiol 2006;56:1657–1661 [CrossRef][PubMed]
    [Google Scholar]
  19. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  20. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  21. Kumar RM, Kaur G, Kumar N, Kumar A, Singh NK et al. Taxonomic description and genome sequence of Salinicoccus sediminis sp. nov., a halotolerant bacterium isolated from marine sediment. Int J Syst Evol Microbiol 2015;65:3794–3799 [CrossRef][PubMed]
    [Google Scholar]
  22. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14:60 [CrossRef][PubMed]
    [Google Scholar]
  23. Lee I, Kim YO, Park SC, Chun J. OrthoANI: An improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016;66:1100–1103 [CrossRef][PubMed]
    [Google Scholar]
  24. Stackebrandt E, Goebel BM. Taxonomic note: a place for dna-dna reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994;44:846–849 [CrossRef]
    [Google Scholar]
  25. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006;33:152–155
    [Google Scholar]
  26. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014;64:346–351 [CrossRef][PubMed]
    [Google Scholar]
  27. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  28. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  29. Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 2004;101:11030–11035 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002474
Loading
/content/journal/ijsem/10.1099/ijsem.0.002474
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error