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Abstract

A Gram-reaction-negative, strictly aerobic, non-motile and rod-shaped bacterium, designated strain BXN5-31, was isolated from soil of a ginseng field, and its taxonomic position was investigated using a polyphasic approach. Strain BXN5-31 grew at 18–37 °C and at pH 6.0–8.0 on R2A medium. Based on 16S rRNA gene sequence similarity, strain BXN5-31 was shown to belong to the genus and was closely related to HME6664, XM-003 and WS71. The DNA G+C content was 43.6 %. The predominant respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids were iso-C, iso-C 3-OH and summed feature 3 (comprising Cω6 and/or Cω7). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The DNA–DNA hybridization values between strain BXN5-31 and three reference strains ( HME6664, XM-003 and WS71) were 9.4±1.9, 8.2±1.3 and 5.7±0.7 %, respectively. The DNA G+C content and chemotaxonomic data supported the affiliation of strain BXN5-31 to the genus . Moreover, the physiological and biochemical results and low level of DNA–DNA relatedness allowed the phenotypic and genotypic differentiation of strain BXN5-31 from recognized species of the genus . The isolate therefore represents a novel species, for which the name sp. nov. is proposed. The type strain is BXN5-31 (=KACC 14957=JCM 17085).

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2018-01-01
2020-01-29
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