1887

Abstract

A novel halophilic bacterium, strain GSS13, capable of growing at salinities of 8–28 % (w/v) NaCl (optimally at 24 %, w/v) was isolated from Yuncheng Saline Lake in China. GSS13 was Gram-stain-positive, strictly aerobic, rod-shaped, motile and a non-spore-former. Growth occurred at pH 5.5–8.5 (optimum pH 7.0) and at 10–45 °C (optimum 30 °C). On the basis of the results of 16S rRNA gene sequences phylogenetic analyses, GSS13 represents a member of the genus and is closely related to S7, CM1 and MSS4, with 16S rRNA gene sequence similarities of 98.7, 98.4 and 97.9 %, respectively. The results of DNA–DNA pairing studies revealed that GSS13 displayed 52, 43 and 48 % relatedness to S7, CM1 and MSS4, respectively. The polar lipids of GSS13 consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol unidentified glycolipids, an unidentified phospholipid and an unidentified lipid. The predominant isoprenoid quinone was MK-7, and the major fatty acids were anteiso-C (32.0 %) and anteiso C (26.4 %). The DNA G+C content of the type strain was 52.1 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, a novel species of the genus is proposed, with the name sp. nov. The type strain is GSS13 (=KCTC 33792=MCCC 1K00567).

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2018-01-01
2024-03-29
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