@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002464, author = "Dai, Yu-Mei and Zhang, Ling-Li and Li, Yan and Li, Yu-Qian and Deng, Xiao-Hui and Wang, Ting-Ting and Yang, Feng and Tian, Yong-Qiang and Li, Na and Zhou, Xing-Mei and Liu, Xiao-Feng and Li, Wen-Jun", title = "Characterization of Trichococcus paludicola sp. nov. and Trichococcus alkaliphilus sp. nov., isolated from a high-elevation wetland, by phenotypic and genomic analyses", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "1", pages = "99-105", doi = "https://doi.org/10.1099/ijsem.0.002464", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002464", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Trichococcus alkaliphilus", keywords = "Qinghai-Tibetan Plateau", keywords = "Trichococcus paludicola", keywords = "alkalophiles", keywords = "genomic analysis", keywords = "wetland soil", abstract = "Two psychrotolerant facultative anaerobes, strains B7-2T and B5T, were isolated from the Zoige Wetland on the Qinghai-Tibetan Plateau. The 16S rRNA gene sequences of strains B7-2T and B5T shared high similarity (>99 %) with those of the type strains of the genus Trichococcus, while their digital DNA–DNA hybridization values with each other (49 %) and with the reference type strains (48–23 %) were lower than 70 %, which suggest that they represent two novel species of the genus Trichococcus. Cells of strains B7-2T and B5T were immotile cocci, grew in the temperature range of 4–37 °C (optimum 25 °C) and were alkaliphilic with optimum growth at pH 9.0. The major components of the cellular fatty acids were C16 : 0, anteiso-C17 : 0 and C18 : 0 for strain B7-2T, and C16 : 0, anteiso-C17 : 0, C18 : 1ω9c and C18 : 0 for strain B5T. The genomic DNA G+C contents were 46.0 and 46.7 mol% for strains B7-2T and B5T, respectively. Based on physiological and genomic characteristics, it is suggested that strains B7-2T and B5T represent two novel species within the genus Trichococcus, for which the names Trichococcus paludicola sp. nov. and Trichococcus alkaliphilus sp. nov. are proposed. The type strains are B7-2T (=DSM 104691T=KCTC 33886T) and B5T (=DSM 104692T=KCTC 33885T), respectively.", }