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Volume 68, Issue 1

sp. nov., isolated from freshwater and emended description of Free

Abstract

A Gram-stain-negative, yellow, facultatively-anaerobic, short, rod-shaped, non-spore-forming bacterium, designated PK15, was isolated from freshwater. Growth was observed at 4–40 °C (optimum, 30 °C), pH 6–9 (optimum, 8), and in the presence of 0–0.8 % (w/v) NaCl (optimum, 0.4 %). Strain PK15 exhibited both catalase and oxidase activities and was able to reduce nitrate. On the basis of 16S rRNA gene sequence similarities, strain PK15 was shown to belong to the genus with close similarities to S44 (97.9 %) and EM1321 (97.7 %). Menaquinone-6 (MK-6) was the major respiratory quinone, while the G+C content of the genomic DNA was 35.5 (±0.9) mol%. The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids, one unidentified aminophospholipid and three unidentified polar lipids. The predominant cellular fatty acids (≥10 %) were anteiso-C (17.3 %), a summed feature comprising Cω7 and/or Cω6 (15.1 %) and iso-C (10.0 %). Chemotaxonomic data supported the affiliation of strain PK15 to the genus . The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain PK15 from strains of closely related species. It was, therefore, evident that PK15 represents a novel species of the genus , for which the name sp. nov. is proposed with strain PK15 (=KCTC 52562=JCM 32115) as the type strain. Based on the results of the chemotaxonomic characterization in the present study, an emended description of is also proposed.

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  • Accepted:
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Keyword(s): Flavobacterium commune , Flavobacterium seoulense and freshwater
© 2018 IUMS
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2018-01-01
2024-04-19
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Flavobacterium commune sp. nov., isolated from freshwater and emended description of Flavobacterium seoulense
Int J Syst Evol Microbiol 68, 93 (2018); https://doi.org/10.1099/ijsem.0.002463
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