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Volume 67, Issue 12

sp. nov. and sp. nov., isolated from a freshwater green alga Free

Abstract

Two Gram-stain-negative, aerobic, catalase- and oxidase-positive, yellow-pigmented bacteria, designated strains Pch-B and Pch-E, were isolated from a green alga . Both strains were motile short rods with a flagellum and optimally grew at pH 6.0–7.0, 25–30 °C and 0–1 % NaCl. They contained ubiquinone-10 as the major quinone, spermidine as the major polyamine, C, C 2-OH and summed features 3 (comprising C 7 and/or C 6) and 8 (comprising C 7 and/or C 6) as the major fatty acids and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidyl--methylethanolamine, two sphingoglycolipids and phosphatidylcholine as the major polar lipids. DNA G+C contents of strains Pch-B and Pch-E were 61.4 and 63.9 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains Pch-B and Pch-E formed a phylogenic lineage with 301. Strains Pch-B and Pch-E had a 99.86 % 16S rRNA gene sequence similarity and their DNA–DNA hybridization (DDH) relatedness value was 40.6 %. Strains Pch-B and Pch-E were most closely related to 301 with 99.93 and 99.76 % 16S rRNA gene sequence similarities, respectively and the DDH values between strains Pch-B and Pch-E and the type strain of were 43.3 and 32.1 %, respectively. In conclusion, strains Pch-B and Pch-E represent novel species of the genus , for which the names sp. nov. and sp. nov. are proposed, respectively. The type strains of and are Pch-B (=KACC 19283=JCM 32054) and Pch-E (=KACC 19284=JCM 32055), respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): green alga , new taxa , Paulinella chromatophora , Sphingobium algicola and Sphingobium paulinellae
© 2017 IUMS
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2017-12-01
2024-04-19
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References

  1. Yabuuchi E, Yano I, Oyaizu H, Hashimoto Y, Ezaki T et al. Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas . Microbiol Immunol 1990; 34:99–119 [View Article][PubMed]
     [Google Scholar]
  2. Takeuchi M, Hamana K, Hiraishi A. Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 2001; 51:1405–1417 [View Article][PubMed]
     [Google Scholar]
  3. Yabuuchi E, Kosako Y, Fujiwara N, Naka T, Matsunaga I et al. Emendation of the genus Sphingomonas Yabuuchi et al. 1990 and junior objective synonymy of the species of three genera, Sphingobium, Novosphingobium and Sphingopyxis, in conjunction with Blastomonas ursincola . Int J Syst Evol Microbiol 2002; 52:1485–1496 [View Article][PubMed]
     [Google Scholar]
  4. Lee JC, Kim SG, Whang KS. Sphingobium subterraneum sp. nov., isolated from ground water. Int J Syst Evol Microbiol 2015; 65:393–398 [View Article][PubMed]
     [Google Scholar]
  5. Chen H, Jogler M, Rohde M, Klenk HP, Busse HJ et al. Sphingobium limneticum sp. nov. and Sphingobium boeckii sp. nov., two freshwater planktonic members of the family Sphingomonadaceae, and reclassification of Sphingomonas suberifaciens as Sphingobium suberifaciens comb. nov. Int J Syst Evol Microbiol 2013; 63:735–743 [View Article][PubMed]
     [Google Scholar]
  6. Lin SY, Shen FT, Lai WA, Zhu ZL, Chen WM et al. Sphingomonas formosensis sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from agricultural soil. Int J Syst Evol Microbiol 2012; 62:1581–1586 [View Article][PubMed]
     [Google Scholar]
  7. Kaur J, Moskalikova H, Niharika N, Sedlackova M, Hampl A et al. Sphingobium baderi sp. nov., isolated from a hexachlorocyclohexane dump site. Int J Syst Evol Microbiol 2013; 63:673–678 [View Article][PubMed]
     [Google Scholar]
  8. Chen X, Wang H, Xu J, Song D, Sun G et al. Sphingobium hydrophobicum sp. nov., a hydrophobic bacterium isolated from electronic-waste-contaminated sediment. Int J Syst Evol Microbiol 2016; 66:3912–3916 [View Article][PubMed]
     [Google Scholar]
  9. Cai S, Shi C, Zhao JD, Cao Q, He J et al. Sphingobium phenoxybenzoativorans sp. nov., a 2-phenoxybenzoic-acid-degrading bacterium. Int J Syst Evol Microbiol 2015; 65:1986–1991 [View Article][PubMed]
     [Google Scholar]
  10. Lee HJ, Jeong SE, Cho MS, Kim S, Lee SS et al. Flavihumibacter solisilvae sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 2014; 64:2897–2901 [View Article][PubMed]
     [Google Scholar]
  11. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  12. Nawrocki EP, Eddy SR. Query-dependent banding (QDB) for faster RNA similarity searches. PLoS Comput Biol 2007; 3:e56 [View Article][PubMed]
     [Google Scholar]
  13. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30:1312–1313 [View Article][PubMed]
     [Google Scholar]
  14. Felsenstein J. PHYLIP (Phylogeny Inference Package), Version 3.6a Seattle, WA: Department of genetics, University of Washington; 2002
     [Google Scholar]
  15. Brosius J, Palmer ML, Kennedy PJ, Noller HF. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci USA 1978; 75:4801–4805 [View Article][PubMed]
     [Google Scholar]
  16. Chang HW, Nam YD, Jung MY, Kim KH, Roh SW et al. Statistical superiority of genome-probing microarrays as genomic DNA-DNA hybridization in revealing the bacterial phylogenetic relationship compared to conventional methods. J Microbiol Methods 2008; 75:523–530 [View Article][PubMed]
     [Google Scholar]
  17. Stackebrandt E, Frederiksen W, Garrity GM, Grimont PA, Kämpfer P et al. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 2002; 52:1043–1047 [View Article][PubMed]
     [Google Scholar]
  18. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006; 33:152–155
     [Google Scholar]
  19. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
     [Google Scholar]
  20. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19:1–67
     [Google Scholar]
  21. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P. (editor) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
     [Google Scholar]
  22. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–208 [Crossref]
     [Google Scholar]
  23. Busse H-J, Bunka S, Hensel A, Lubitz W. Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 1997; 47:698–708 [View Article]
     [Google Scholar]
  24. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  25. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002; 4:770–773[PubMed] [Crossref]
     [Google Scholar]
  26. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977; 27:104–117 [View Article]
     [Google Scholar]
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Sphingobium paulinellae sp. nov. and Sphingobium algicola sp. nov., isolated from a freshwater green alga Paulinella chromatophora
Int J Syst Evol Microbiol 67, 5165 (2017); https://doi.org/10.1099/ijsem.0.002435
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