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Abstract

Two Gram-stain-negative, aerobic, catalase- and oxidase-positive, yellow-pigmented bacteria, designated strains Pch-B and Pch-E, were isolated from a green alga Paulinella chromatophora. Both strains were motile short rods with a flagellum and optimally grew at pH 6.0–7.0, 25–30 °C and 0–1 % NaCl. They contained ubiquinone-10 as the major quinone, spermidine as the major polyamine, C16 : 0, C14 : 0 2-OH and summed features 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c) and 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c) as the major fatty acids and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, two sphingoglycolipids and phosphatidylcholine as the major polar lipids. DNA G+C contents of strains Pch-B and Pch-E were 61.4 and 63.9 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains Pch-B and Pch-E formed a phylogenic lineage with Sphingobium limneticum 301. Strains Pch-B and Pch-E had a 99.86 % 16S rRNA gene sequence similarity and their DNA–DNA hybridization (DDH) relatedness value was 40.6 %. Strains Pch-B and Pch-E were most closely related to S. limneticum 301 with 99.93 and 99.76 % 16S rRNA gene sequence similarities, respectively and the DDH values between strains Pch-B and Pch-E and the type strain of S. limneticum were 43.3 and 32.1 %, respectively. In conclusion, strains Pch-B and Pch-E represent novel species of the genus Sphingobium , for which the names Sphingobium paulinellae sp. nov. and Sphingobium algicola sp. nov. are proposed, respectively. The type strains of S. paulinellae and S. algicola are Pch-B (=KACC 19283=JCM 32054) and Pch-E (=KACC 19284=JCM 32055), respectively.

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2019-10-15
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