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Abstract

A phthalate esters-degrading bacterial strain, designated QH-11, was isolated from an activated sludge wastewater treatment plant in Beijing, PR China. The cells were aerobic, Gram-stain-positive, non-motile, catalase-positive, oxidase-negative, short rods and formed white colonies on trypticase soy agar. This isolate contained -diaminopimelic acid as the diagnostic diamino acid and whole-cell hydrolysates contained arabinose and ribose. Diphosphatidylglycerol and phosphatidylethanolamine were the predominant polar lipids. According to the results of full-length of 16S rRNA gene sequence analysis, QH-11 represented a member of the genus and showed the highest sequence similarity to DSM 44015 (99.2 %), but was distinguishable by a low level of DNA–DNA relatedness (37.8 %). Genome-based comparisons indicated a clear distinction from the top ten most similar type strains (16S rRNA gene sequence) with pairwise average nucleotide identities (ANI) between 74.6 and 83.4 %. The predominant respiratory quinone was MK-9(H), the mycolic acids present had 56 to 62 carbon atoms, and the major fatty acids were C (33.3 %), Cω8 (23.4 %) and Cω9 (17.9 %). The DNA G+C content was 68.0 mol%. On the basis of the results of DNA–DNA hybridization, ANI and physiological and biochemical tests, it is proposed that QH-11 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is QH-11 (CICC 24107 =KCTC 39933).

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2017-12-01
2020-01-27
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