1887

Abstract

Strain K-4-11-1, a psychrotolerant, light salmon-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, was isolated from forest soil of Kyonggi University, Suwon, South Korea. It was able to grow at 0–32 °C, at pH 5.0–10.0 and with 0–1.5 % (w/v) NaCl. This strain was taxonomically characterized by a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain K-4-11-1 belongs to the genus Pedobacter and is closely related to Pedobacter ginsenosidimutans THG-45 (98.75 % sequence similarity), Pedobacter borealis G-1 (98.48 %), Pedobacter terrae DS-57 (98.20 %), Pedobacter agri PB92 (97.92 %) and Pedobacter suwonensis 15-52 (97.84 %). The only respiratory quinone was menaquinone-7. The major polar lipids were phosphatidylethanolamine and unidentified glycolipids. The predominant fatty acids of strain K-4-11-1 were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0, iso-C17 : 0 3-OH, anteiso-C17 : 0 and summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl). The genomic DNA G+C content of this novel strain was 38.3 mol%. The DNA–DNA relatedness values between strain K-4-11-1 and P. ginsenosidimutans KACC 14530, P. borealis KACC 14287, P. terrae KACC 13766, P. agri KACC 13768 and P. suwonensis KACC 11317 were 40.0, 36.3, 37.0, 32.3 and 29.7 %, respectively. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this novel strain from its closest phylogenetic neighbours. Thus, strain K-4-11-1 represents a novel species of the genus Pedobacter , for which the name Pedobacter kyonggii sp. nov. is proposed. The type strain is K-4-11-1 (=KEMB 9005-574=KACC 19174=JCM 31916).

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2017-10-19
2019-10-20
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