RT Journal Article SR Electronic(1) A1 Chaudhary, Dhiraj Kumar A1 Dahal, Ram Hari A1 Kim, JaisooYR 2018 T1 Nemorella caseinilytica gen. nov., sp. nov., isolated from forest soil JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 2 SP 474 OP 481 DO https://doi.org/10.1099/ijsem.0.002427 PB Microbiology Society, SN 1466-5034, AB Two novel strains, J116-2T and J116-1, were isolated from forest soil and were taxonomically characterized by a polyphasic approach. Both strains were yellow-coloured, Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacteria. The strains were non-sporulating, catalase-positive and oxidase-negative. Strains J116-2T and J116-1 were able to grow at 20–32 °C, pH 6.0–8.5, and 0–0.5 % (w/v) NaCl concentration. Based on 16S rRNA gene sequence analyses, strains J116-2T and J116-1 formed a distinct lineage within the family Chitinophagaceae of the phylum Bacteroidetes and were closely related to genera Taibaiella (89.86–89.30 % sequence similarity), Falvihumibacter (89.20–89.06 %), Filimonas (89.06 %) and Chitinophaga (89.01–88.77 %). The pairwise sequence similarity between strains J116-2T and J116-1 was found to be 99.86 %. Flexirubin-type pigments were absent in both strains. The only respiratory quinone was menaquinone-7 (MK-7); the major polar lipid was phosphatidylethanolamine; the predominant polyamine was homospermidine; and the major fatty acids were C15 : 0iso, C15 : 1iso G and C17 : 0iso 3-OH. The genomic DNA G+C content values of strains J116-2T and J116-1 were 51.1 and 50.9 mol%, respectively. On the basis of phenotypic, genotypic and phylogenetic analysis, strain J116-2T represents a novel species of a new genus in the family Chitinophagaceae , for which the name Nemorella caseinilytica gen. nov., sp. nov. is proposed. The type strain of Nemorella caseinilytica is J116-2T (=KEMB 9005–550T=KACC 19168T=NBRC 112827T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002427