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Abstract

A Gram stain-negative, motile and rod-shaped bacterial strain, designated NB097-1, was isolated from a marine sediment sample collected from the Bering Sea, and subjected to a polyphasic taxonomic study. Strain NB097-1 grew at 0–25 °C, with an optimum growth temperature of 10–13 °C. Phylogenetic trees reconstructed based on 16S rRNA gene sequences indicated that strain NB097-1 belonged to the genus . Strain NB097-1 exhibited 16S rRNA gene sequence similarities of 98.6, 98.5, 98.0, 97.2 and 96.8 % with the type strains of , , , and , respectively. Strain NB097-1 had Q-8 as the major respiratory quinone and contained summed feature 3 (C 7 and/or C 6) and C as the major fatty acids. The major polar lipids detected in strain NB097-1 were phosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content of strain NB097-1 was 38.5 mol%, and its average nucleotide identity values with the type strains of , , , and were 77.30, 78.99, 78.82, 80.66 and 75.77 %, respectively. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, together with average nucleotide identity value data, strain NB097-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NB097-1 (=MCCC 1A11668=KCTC 52554).

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2017-12-01
2020-01-25
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References

  1. Deming JW, Somers LK, Straube WL, Swartz DG, Macdonell MT. Isolation of an obligately barophilic bacterium and description of a new genus, Colwellia gen. nov. Syst Appl Microbiol 1988;10:152–160 [CrossRef]
    [Google Scholar]
  2. Bowman JP, Gosink JJ, McCammon SA, Lewis TE, Nichols DS et al. Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22:ω63). Int J Syst Bacteriol 1998;48:1171–1180 [CrossRef]
    [Google Scholar]
  3. Anzai Y, Kim H, Park JY, Wakabayashi H, Oyaizu H. Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 2000;50:1563–1589 [CrossRef][PubMed]
    [Google Scholar]
  4. Yu Y, Li HR, Zeng YX. Colwellia chukchiensis sp. nov., a psychrotolerant bacterium isolated from the Arctic Ocean. Int J Syst Evol Microbiol 2011;61:850–853 [CrossRef][PubMed]
    [Google Scholar]
  5. Nogi Y, Hosoya S, Kato C, Horikoshi K. Colwellia piezophila sp. nov., a novel piezophilic species from deep-sea sediments of the Japan Trench. Int J Syst Evol Microbiol 2004;54:1627–1631 [CrossRef][PubMed]
    [Google Scholar]
  6. Zhang DC, Yu Y, Xin YH, Liu HC, Zhou PJ et al. Colwellia polaris sp. nov., a psychrotolerant bacterium isolated from Arctic sea ice. Int J Syst Evol Microbiol 2008;58:1931–1934 [CrossRef][PubMed]
    [Google Scholar]
  7. Smibert RM, Krieg NR. Phenotypic characterization. In: Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  8. Dong XZ, Cai MY. Determination of biochemical properties. In: Manual for the Systematic Identification of General Bacteria Beijing: Science Press; 2001; pp.370–398
    [Google Scholar]
  9. Yurkov V, Stackebrandt E, Holmes A, Fuerst JA, Hugenholtz P et al. Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Bacteriol 1994;44:427–434 [CrossRef][PubMed]
    [Google Scholar]
  10. Leifson E. Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 1963;85:1183[PubMed]
    [Google Scholar]
  11. Shieh WY, Chen YW, Chaw SM, Chiu HH. Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 2003;53:479–484 [CrossRef][PubMed]
    [Google Scholar]
  12. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406[PubMed]
    [Google Scholar]
  13. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  14. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  15. Komagata K, Suzuki KI. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1988;19:161–207[Crossref]
    [Google Scholar]
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI; 1990
    [Google Scholar]
  17. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  18. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009;106:19126–19131 [CrossRef][PubMed]
    [Google Scholar]
  19. Jung SY, Oh TK, Yoon JH. Colwellia aestuarii sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 2006;56:33–37 [CrossRef][PubMed]
    [Google Scholar]
  20. Kim YO, Park IS, Park S, Nam BH, Jung YT et al. Colwellia mytili sp. nov., isolated from mussel Mytilus edulis. Int J Syst Evol Microbiol 2017;67:31–36 [CrossRef][PubMed]
    [Google Scholar]
  21. Park S, Jung YT, Yoon JH. Colwellia sediminilitoris sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2016;66:3258–3263 [CrossRef][PubMed]
    [Google Scholar]
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