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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 67, Issue 12

sp. nov., isolated from rhizosphere soil of a tomato plant Free

Abstract

The taxonomic position of a bacterial strain designated T16R-228, isolated from a rhizosphere soil sample of a tomato plant collected from a farm in Buyeo, Chungcheongnam-do, Republic of Korea, was determined using a polyphasic approach. On the basis of morphological, genetic and chemotaxonomic characteristics, it was determined to belong to the genus . It was an aerobic, Gram-stain-positive, non-motile, catalase-negative, oxidase-negative rod with peritrichous flagella. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, hydroxyl- phosphatidylethanolamine and one unidentified polar lipid. Menaquiones were MK-7. Predominant cellular fatty acids were anteiso-C, C and iso-C. DNA G+C content was 56.8 mol%. The phylogenetic tree constructed based on the 16S rRNA gene sequences showed the strain formed a clade with VKPM B-7519, T7, KCTC 3748, YC300, B27 and SD17, showing the highest sequence similarity with VKPM B-7519 (96.5 %). The polyphasic data supported that strain T16R-228 was clearly distinguished from its closely related species and represents a novel species of the genus for which the name is proposed. The type strain is T16R-228 (=KACC 18654=NBRC 111896).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Paenibacillus solanacearum and polar lipid
© 2017 IUMS
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2017-12-01
2025-01-26
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References

  1. Ash C, Priest FG, Collins MD. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus . Antonie van Leeuwenhoek 1993; 64:253–260[PubMed] [Crossref]
     [Google Scholar]
  2. Ludwig W, Schleifer KH, Whitman WB. Family IV. Paenibacillaceae fam. nov. In de Vos P, Garrity GM, Jones D, Krieg NR, Ludwig W et al. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 3 New York: Springer; 2009 pp. 269
     [Google Scholar]
  3. Cao Y, Chen F, Li Y, Wei S, Wang G. Paenibacillus ferrarius sp. nov., isolated from iron mineral soil. Int J Syst Evol Microbiol 2015; 65:165–170 [View Article][PubMed]
     [Google Scholar]
  4. Chou JH, Chou YJ, Lin KY, Sheu SY, Sheu DS et al. Paenibacillus fonticola sp. nov., isolated from a warm spring. Int J Syst Evol Microbiol 2007; 57:1346–1350 [View Article][PubMed]
     [Google Scholar]
  5. Yao R, Wang R, Wang D, Su J, Zheng S et al. Paenibacillus selenitireducens sp. nov., a selenite-reducing bacterium isolated from a selenium mineral soil. Int J Syst Evol Microbiol 2014; 64:805–811 [View Article][PubMed]
     [Google Scholar]
  6. Priest FG. Genus I. Paenibacillus . In de Vos P, Garrity GM, Jones D, Krieg NR, Ludwig W et al. (editors) Bergy’s Manual of Systematic Bacteriology, 2nd ed. vol. 3 New York: Springer; 2009 pp. 269–295
     [Google Scholar]
  7. Gregersen T. Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 1978; 5:123–127 [View Article]
     [Google Scholar]
  8. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [View Article][PubMed]
     [Google Scholar]
  9. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  10. Ludwig W, Strunk O, Westram R, Richter L, Meier H et al. ARB: a software environment for sequence data. Nucleic Acids Res 2004; 32:1363–1371 [View Article][PubMed]
     [Google Scholar]
  11. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  12. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993; 10:512–526[PubMed]
     [Google Scholar]
  13. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
     [Google Scholar]
  14. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  15. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
     [Google Scholar]
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  17. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  18. Collins MD, Goodfellow M, Minnikin DE. Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curtobacterium and related taxa. J Gen Microbiol 1980; 118:29–37 [View Article][PubMed]
     [Google Scholar]
  19. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002; 4:770–773 [View Article][PubMed]
     [Google Scholar]
  20. Khianngam S, Tanasupawat S, Akaracharanya A, Kim KK, Lee KC et al. Paenibacillus xylanisolvens sp. nov., a xylan-degrading bacterium from soil. Int J Syst Evol Microbiol 2011; 61:160–164 [View Article][PubMed]
     [Google Scholar]
  21. Kim DS, Bae CY, Jeon JJ, Chun SJ, Oh HW et al. Paenibacillus elgii sp. nov., with broad antimicrobial activity. Int J Syst Evol Microbiol 2004; 54:2031–2035 [View Article][PubMed]
     [Google Scholar]
  22. Hu XF, Li SX, Wu JG, Wang JF, Fang QL et al. Transfer of Bacillus mucilaginosus and Bacillus edaphicus to the genus Paenibacillus as Paenibacillus mucilaginosus comb. nov. and Paenibacillus edaphicus comb. nov. Int J Syst Evol Microbiol 2010; 60:8–14 [View Article][PubMed]
     [Google Scholar]
  23. Kuroshima K-I, Sakane T, Takata R, Yokota A. Bacillus ehimensis sp. nov. and Bacillus chitinolyticus sp. nov., new chitinolytic members of the genus Bacillus . Int J Syst Bacteriol 1996; 46:76–80 [View Article]
     [Google Scholar]
  24. Kim DS, Bae CY, Jeon JJ, Chun SJ, Oh HW et al. Paenibacillus elgii sp. nov., with broad antimicrobial activity. Int J Syst Evol Microbiol 2004; 54:2031–2035 [View Article][PubMed]
     [Google Scholar]
  25. Chung YR, Kim CH, Hwang I, Chun J. Paenibacillus koreensis sp. nov., a new species that produces an iturin-like antifungal compound. Int J Syst Evol Microbiol 2000; 50:1495–1500 [View Article][PubMed]
     [Google Scholar]
  26. Wu X, Fang H, Qian C, Wen Y, Shen X et al. Paenibacillus tianmuensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 2011; 61:1133–1137 [View Article][PubMed]
     [Google Scholar]
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Paenibacillus solanacearum sp. nov., isolated from rhizosphere soil of a tomato plant
Int J Syst Evol Microbiol 67, 5046 (2017); https://doi.org/10.1099/ijsem.0.002410
/content/journal/ijsem/10.1099/ijsem.0.002410
/content/journal/ijsem/10.1099/ijsem.0.002410
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