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Abstract

A novel actinomycete, designated strain NEAU-BB2C19, was isolated from the root of black soya bean [ (L.) Merr] collected from Harbin, Heilongjiang Province, China, and characterized using a polyphasic approach. The strain was an aerobic, Gram-stain-positive actinomycete that formed extensively branched substrate mycelium and aerial hyphae. The predominant menaquinones were MK-9(H) and MK-9(H). The major cellular fatty acid profile consisted of iso-C, 10-methyl C and 10-methyl C. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, phosphatidylglycerol and glycolipid. The DNA G+C content was 68.2±0.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-BB2C19 should be assigned to the genus and formed a distinct branch with its closest neighbour NEAU-ZJ3 (98.75 % 16S rRNA gene sequence similarity). The morphological and chemotaxonomic properties of the strain were also consistent with those of members of the genus A combination of DNA–DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-BB2C19 could be clearly differentiated from its closest phylogenetic relative. Thus, the strain is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-BB2C19 (=CGMCC 4.7430=DSM 104838).

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2017-12-01
2022-01-27
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