RT Journal Article SR Electronic(1) A1 Kim, Caroline C. A1 Kelly, William J. A1 Patchett, Mark L. A1 Tannock, Gerald W. A1 Jordens, Z. A1 Stoklosinski, Halina M. A1 Taylor, Jordan W. A1 Sims, Ian M. A1 Bell, Tracey J. A1 Rosendale, Douglas I.YR 2017 T1 Monoglobus pectinilyticus gen. nov., sp. nov., a pectinolytic bacterium isolated from human faeces JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 12 SP 4992 OP 4998 DO https://doi.org/10.1099/ijsem.0.002395 PB Microbiology Society, SN 1466-5034, AB A novel anaerobic pectinolytic bacterium (strain 14T) was isolated from human faeces. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 14T belonged to the family Ruminococcaceae , but was located separately from known clostridial clusters within the taxon. The closest cultured relative of strain 14T was Acetivibrio cellulolyticus (89.7 % sequence similarity). Strain 14T shared ~99 % sequence similarity with cloned 16S rRNA gene sequences from uncultured bacteria derived from the human gut. Cells were Gram-stain-positive, non-motile cocci approximately 0.6 µm in diameter. Strain 14T fermented pectins from citrus peel, apple, and kiwifruit as well as carbohydrates that are constituents of pectins and hemicellulose, such as galacturonic acid, xylose, and arabinose. TEM images of strain 14T, cultured in association with plant tissues, suggested extracellular fibrolytic activity associated with the bacterial cells, forming zones of degradation in the pectin-rich regions of middle lamella. Phylogenetic and phenotypic analysis supported the differentiation of strain 14T as a novel genus in the family Ruminococcaceae . The name Monoglobus pectinilyticus gen. nov., sp. nov. is proposed; the type strain is 14T (JCM 31914T=DSM 104782T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002395