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Abstract

A novel Gram-stain-negative, rod-shaped, non-motile and red-pigmented bacterial strain, designated K1E01-27, was isolated from an animal excrement sample which was found in a karst cave located in Guizhou province, China. The phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K1E01-27 represented a member of the genus Hymenobacter within the family Cytophagaceae of the phylum Bacteroidetes . Strain K1E01-27 was most closely related to Hymenobacter algoricola VUG-A23a, with 95.1 % 16S rRNA gene sequence similarity. Growth of strain K1E01-27 occurred at 4–35 °C, at pH 5–9 and in the presence of 0–0.5 % (w/v) NaCl. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 1ω5c, summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 4 (comprising iso-C17 : 1 I/anteiso-C17 : 1 B). The major isoprenoid quinone was menaquinone 7 (MK-7). The major polar lipids were phosphatidylethanolamine, five unidentified aminophospholipids, three unidentified glycolipids, two unidentified phospholipids and one unidentified polar lipid. The DNA G+C content of the genomic DNA was 54.9 mol%. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain K1E01-27 is considered to represent a novel species of the genus Hymenobacter , for which the name Hymenobacter cavernae sp. nov. is proposed. The type strain is K1E01-27 (=CGMCC 1.15197=NBRC 112610).

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2017-10-06
2019-10-20
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