1887

Abstract

A novel strain, designated EM302, was isolated from a soil sample collected from farmland located in Yesan-gun in the Republic of Korea. The cells were Gram-stain-negative, aerobic, non-motile without flagella and coccobacilli-shaped. Colonies were circular with entire edges, convex, opaque and pink. The strain grew at 15–40 ˚C (optimum, 35 ˚C) and pH 6.0–9.0 (optimum, 7.0). The strain grew in R2A medium without the addition of NaCl. Based on 16S rRNA gene sequences analysis, strain EM302 was found to be phylogenetically related to WW53 (96.5 % 16S rRNA gene sequence similarity), MC 3624 (95.2 %), 5420-30 (94.5 %), N75 (94.4 %) and CPCC 100056 (94.4 %). The major cellular fatty acids were summed feature 8 (C 7 and/or C 6) and C. The predominant respiratory quinone was Q-10. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminolipid. The DNA G+C content of the type strain was 65.7 mol%. On the basis of data presented here, strain EM302 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EM302 (=KACC 13942=KCTC 42906=NBRC 111477).

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2017-11-01
2019-12-13
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