1887

Abstract

A taxonomic study was carried out on strain L54-1-50, which was isolated from surface seawater of the South China Sea. Cells of strain L54-1-50 were Gram-stain-negative, rod-shaped, oxidase-positive and catalase-positive. Growth was observed at salinities from 0 to 9 % (optimum 2 %, w/v), at pH 6.0–10.0 (optimum 8.0–9.0) and at temperatures from 10 to 45 °C (optimum 25–37 °C), but not at 4 or 50 °C. The 16S rRNA gene sequence analysis indicated that strain L54-1-50 was a member of the genus Oceanibaculum , related to Oceanibaculum indicum P24 (98.8 %) and Oceanibaculum pacificum MC2UP-L3 (97.7 %). The digital DNA–DNA hybridization values between strain L54-1-50 and the two type strains O. indicum P24 and O. pacificum MC2UP-L3 were 35.4±2.5 and 23.7±2.5 %, respectively. The average nucleotide identity values between strain L54-1-50 and two type strains were 79.7 and 88.3 %, respectively. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0 and C18 : 1 2-OH. The respiratory quinone was Q-10. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, two unidentified phospholipids and three unidentified lipids. The G+C content of the chromosomal DNA was 65.1 mol%. The combined genotypic and phenotypic data showed that strain L54-1-50 represents a novel species of the genus Oceanibaculum , for which the name Oceanibaculum nanhaiense sp. nov. is proposed, with the type strain L54-1-50 (=KCTC 52312=MCCC 1A05150).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002388
2017-10-12
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/11/4842.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002388&mimeType=html&fmt=ahah

References

  1. Pfennig N, Truper HG. Higher taxa of the phototrophic bacteria. Int J Syst Bacteriol 1971;21:17–18 [CrossRef]
    [Google Scholar]
  2. Lai Q, Yuan J, Wu C, Shao Z. Oceanibaculum indicum gen. nov., sp. nov., isolated from deep seawater of the Indian Ocean. Int J Syst Evol Microbiol 2009;59:1733–1737 [CrossRef][PubMed]
    [Google Scholar]
  3. Dong C, Lai Q, Chen L, Sun F, Shao Z et al. Oceanibaculum pacificum sp. nov., isolated from hydrothermal field sediment of the South-West Pacific Ocean. Int J Syst Evol Microbiol 2010;60:219–222 [CrossRef][PubMed]
    [Google Scholar]
  4. Ausubel FM, Brent R, Kingston RE, Moore DH, Seidman JG et al. Short Protocols in Molecular Biology: (3rd Edition). A Compendium of Methods from “Current Protocols in Molecular Biology”. New York: John Wiley & Sons, Inc.; 1995; p.356
    [Google Scholar]
  5. Liu C, Shao Z. Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 2005;55:1181–1186 [CrossRef][PubMed]
    [Google Scholar]
  6. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  7. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  8. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  9. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  10. Rzhetsky A, Nei M. Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 1993;10:1073–1095[PubMed]
    [Google Scholar]
  11. Luo R, Liu B, Xie Y, Li Z, Huang W et al. SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Gigascience 2012;1:18 [CrossRef][PubMed]
    [Google Scholar]
  12. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14:60 [CrossRef][PubMed]
    [Google Scholar]
  13. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA–DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007;57:81–91 [CrossRef][PubMed]
    [Google Scholar]
  14. Wayne LG. International Committee on Systematic Bacteriology: announcement of the report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Zentralbl Bakteriol Mikrobiol Hyg A 1988;268:433–434[PubMed]
    [Google Scholar]
  15. Thompson CC, Chimetto L, Edwards RA, Swings J, Stackebrandt E et al. Microbial genomic taxonomy. BMC Genomics 2013;14:913 [CrossRef][PubMed]
    [Google Scholar]
  16. Lai Q, Yuan J, Gu L, Shao Z. Marispirillum indicum gen. nov., sp. nov., isolated from a deep-sea environment. Int J Syst Evol Microbiol 2009;59:1278–1281 [CrossRef][PubMed]
    [Google Scholar]
  17. Mitchell G, Dole R, Sasser JR, Ferguson WC, Lynn JT et al. A national health policy?. Rev Fed Am Health Syst 1990;23:22–35, 38, 42[PubMed]
    [Google Scholar]
  18. Collins MD. Isoprenoid Quinone Analyses in Bacterial Classification and Identification Society for Applied Bacteriology Technical London, UK: Academic Press; 1985.; pp. 267–287
    [Google Scholar]
  19. Kates M. Techniques of Lipidology: Isolation, Analysis and Identification of Lipidsvol. 3 Laboratory Techniques in Biochemistry & Molecular Biology Amsterdam: Elsevier; 1986; pp.0151–0155
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002388
Loading
/content/journal/ijsem/10.1099/ijsem.0.002388
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error