1887

Abstract

Cells of strain SW33, isolated from the seawater of Asan Bay, Republic of Korea, were characterized as Gram-stain-negative, aerobic, rod-shaped, motile and non-spore-forming. Phylogenetic analysis revealed that strain SW33 belonged to the genus Psychrosphaera and clustered distantly with the other genera in the family Pseudoalteromonadaceae in the phylogenetic tree. The 16S rRNA sequences of strain SW33 revealed high similarities to Psychrosphaera saromensis SA4-48 (98.7 %), Psychrosphaera haliotis KDW4 (97.4 %) and Psychrosphaera aestuarii PSC101 (97.3 %). The major fatty acids were C16 : 0 (27.9 %), summed feature 3 (32.2 %) and summed feature 8 (17.2 %). The predominant quinone was Q-8, and the polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified amino lipid. The DNA G+C content was 38.3 mol%. The DNA–DNA relatedness values with the three species of Psychrosphaera saromensis KCTC 23240, Psychrosphaera haliotis KCTC 22500 and Psychrosphaera aestuarii KCTC 32274 were 22, 23 and 18 %, respectively. Based on the phenotypic characteristics and taxonomic analyses, we propose that strain SW33 represents a novel species within the genus Psychrosphaera , for which the name Psychrosphaera aquimarina sp. nov. with the type strain SW33 (=KCTC 52743=CICC 24249) is proposed.

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/content/journal/ijsem/10.1099/ijsem.0.002384
2017-10-06
2019-10-18
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References

  1. Park S, Yoshizawa S, Hamasaki K, Kogure K, Yokota A. Psychrosphaera saromensis gen. nov., sp. nov., within the family Pseudoalteromonadaceae, isolated from Lake Saroma, Japan. J Gen Appl Microbiol 2010;56:475–480 [CrossRef][PubMed]
    [Google Scholar]
  2. Lee JH, Baik KS, Kim D, Seong CN. Psychrosphaera aestuarii sp. nov. and Psychrosphaera haliotis sp. nov., isolated from the marine environment, and emended description of the genus Psychrosphaera. Int J Syst Evol Microbiol 2014;64:1952–1957 [CrossRef][PubMed]
    [Google Scholar]
  3. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  4. Hall TA. Bioedit: a user-friendly biological sequence alignment editor and analysis program for window 95/98/NT. Nucleic Acids Symp Ser 1999;95–98
    [Google Scholar]
  5. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  6. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  7. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  8. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  9. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  10. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  11. Bates RG, Bower VE. Alkaline solutions for pH control. Anal Chem 1956;28:1322–1324 [CrossRef]
    [Google Scholar]
  12. Hamouda T, Shih AY, Baker JR. A rapid staining technique for the detection of the initiation of germination of bacterial spores. Lett Appl Microbiol 2002;34:86–90 [CrossRef][PubMed]
    [Google Scholar]
  13. Hiraishi A, Shin YK, Sugiyama J, Komagata K. Isoprenoid quinones and fatty acids of Zoogloea. Antonie van Leeuwenhoek 1992;61:231–236 [CrossRef][PubMed]
    [Google Scholar]
  14. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  15. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  16. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989;39:224–229 [CrossRef]
    [Google Scholar]
  17. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
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