1887

Abstract

A novel acidophilic heterotrophic bacterium, strain Ok2G, was isolated from a freshwater lake in Japan. Cells of the isolate were Gram-stain-negative and non-motile rods (0.6–0.8×1.0–2.8 µm). Growth was observed at 4–35 °C with an optimum growth temperature of 28 °C. The pH range for growth was 3.0–6.2 with an optimum pH of 4.5. The strain utilized fructose, glucose, sucrose, mannitol, sorbitol, ethanol, benzyl alcohol, pyruvate, yeast extract and tryptone as carbon and energy sources for aerobic growth. DNA G+C content was 62.6 mol%. The major cellular fatty acid and the isoprenoid quinone were summed feature 8 (Cω7 and/or Cω6) and Q-10, respectively. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain Ok2G belongs to the genus but is distinct from existing species with sequence similarities lower than 97 %. On the basis of these results, strain Ok2G (=NBRC 112502=DSM 104037) is proposed as the type strain of a novel species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002376
2017-11-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/11/4773.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002376&mimeType=html&fmt=ahah

References

  1. Kishimoto N, Kosako Y, Wakao N, Tano T, Hiraishi A. Transfer of Acidiphilium facilis and Acidiphilium aminolytica to the genus Acidocella gen. nov., and emendation of the genus Acidiphilium. Syst Appl Microbiol 1995;18:85–91 [CrossRef]
    [Google Scholar]
  2. Kimoto K, Aizawa T, Urai M, Ve NB, Suzuki K et al. Acidocella aluminiidurans sp. nov., an aluminium-tolerant bacterium isolated from Panicum repens grown in a highly acidic swamp in actual acid sulfate soil area of Vietnam. Int J Syst Evol Microbiol 2010;60:764–768 [CrossRef][PubMed]
    [Google Scholar]
  3. Jones RM, Hedrich S, Johnson DB. Acidocella aromatica sp. nov.: an acidophilic heterotrophic alphaproteobacterium with unusual phenotypic traits. Extremophiles 2013;17:841–850 [CrossRef][PubMed]
    [Google Scholar]
  4. Wichlacz PL, Unz RF, Langworthy TA. Acidiphilium angustum sp. nov., Acidiphilium facilis sp. nov., and Acidiphilium rubrum sp. nov.: acidophilic heterotrophic bacteria isolated from acidic coal mine drainage. Int J Syst Bacteriol 1986;36:197–201 [CrossRef]
    [Google Scholar]
  5. Kishimoto N, Kosako Y, Tano T. Acidiphilium aminolytica sp. nov.: an acidophilic chemoorganotrophic bacterium isolated from acidic mineral environment. Curr Microbiol 1993;27:131–136 [CrossRef][PubMed]
    [Google Scholar]
  6. Johnson DB, Rolfe S, Hallberg KB, Iversen E. Isolation and phylogenetic characterization of acidophilic microorganisms indigenous to acidic drainage waters at an abandoned Norwegian copper mine. Environ Microbiol 2001;3:630–637 [CrossRef][PubMed]
    [Google Scholar]
  7. Hallberg KB, Coupland K, Kimura S, Johnson DB. Macroscopic streamer growths in acidic, metal-rich mine waters in north wales consist of novel and remarkably simple bacterial communities. Appl Environ Microbiol 2006;72:2022–2030 [CrossRef][PubMed]
    [Google Scholar]
  8. Lu S, Gischkat S, Reiche M, Akob DM, Hallberg KB et al. Ecophysiology of Fe-cycling bacteria in acidic sediments. Appl Environ Microbiol 2010;76:8174–8183 [CrossRef][PubMed]
    [Google Scholar]
  9. Atlas RM. Handbook of Microbiological Media, 4th ed. Boca Raton, FL: CRC Press; 2010;[Crossref]
    [Google Scholar]
  10. Katayama-Fujimura Y, Komatsu Y, Kuraishi H, Kaneko T. Estimation of DNA basecomposition by high-performance liquid chromatography of its nuclease P1 hydrolysate. Agric Biol Chem 1984;48:3169–3172
    [Google Scholar]
  11. Wakao N, Nagasawa N, Matsuura T, Matsukura H, Matsumoto T et al. Acidiphilium multivorum sp. nov., an acidophilic chemoorganotrophic bacterium from pyritic acid mine drainage. J Gen Appl Microbiol 1994;40:143–159 [CrossRef]
    [Google Scholar]
  12. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991; pp.115–175
    [Google Scholar]
  13. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007;23:2947–2948 [CrossRef][PubMed]
    [Google Scholar]
  14. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002376
Loading
/content/journal/ijsem/10.1099/ijsem.0.002376
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error