1887

Abstract

A novel marine bacterial strain, designated JAM 119, was isolated from a tubeworm trophosome in Kagoshima Bay, Japan. Cells were Gram-negative, rod-shaped, non-spore-forming aerobic chemoorganotrophs and motile by means of a single polar flagellum. The isolate grew optimally at 25–27 °C and in the presence of 3 % NaCl. The major respiratory quinone was Q-10. The predominant fatty acid was C18 : 1ω7c. Phosphatidylcholine, phosphatidylglycerol and an unidentified aminolipid were the major polar lipids. On the basis of 16S rRNA gene sequence analysis, the isolated strain was closely affiliated with members of the genus Planktotalea in the class Alphaproteobacteria , and the 16S rRNA gene sequence similarity of the new isolates with the closest related species, Planktotalea frisia SH6-1, was 97.3 %. The DNA G+C content of the novel strain was 57.0 mol%. Based on differences in taxonomic characteristics, the isolated strain represents a novel species of the genus Planktotalea , for which the name Planktotalea lamellibrachiae sp. nov. (type strain JAM 119; JCM 31859=DSMZ 104669) is proposed.

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2017-10-06
2019-09-22
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References

  1. Hahnke S, Tindall BJ, Schumann P, Sperling M, Brinkhoff T et al. Planktotalea frisia gen. nov., sp. nov., isolated from the southern North Sea. Int J Syst Evol Microbiol 2012; 62: 1619– 1624 [CrossRef] [PubMed]
    [Google Scholar]
  2. Buchan A, González JM, Moran MA. Overview of the marine Rose obacter lineage. Appl Environ Microbiol 2005; 71: 5665– 5677 [CrossRef] [PubMed]
    [Google Scholar]
  3. Garrity GM, Bell JA, Lilburn T. Family I. Rhodobacteraceae fam. nov. Bergey's Manual of Systematic Bacteriology. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) The Proteobacteria, part C, The Alpha-, Beta-, Delta-, and Epsilonproteobacteria, 2nd ed.vol. 2 New York: Springer; 2005; pp. 161
    [Google Scholar]
  4. Miura T, Nedachi M, Hashimoto J. Sulphur sources for chemoautotrophic nutrition of shallow water vestimentiferan tubeworms in Kagoshima Bay. J Mar Biol Assoc UK 2002; 82: 537– 540 [CrossRef]
    [Google Scholar]
  5. Barrow GI, Feltham RKA. Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd ed. New York, NY: Cambridge University Press; 1993; [Crossref]
    [Google Scholar]
  6. Baumann L, Baumann P, Mandel M, Allen RD. Taxonomy of aerobic marine eubacteria. J Bacteriol 1972; 110: 402– 429 [PubMed]
    [Google Scholar]
  7. Saito H, Miura KI. Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 1963; 72: 619– 629 [CrossRef] [PubMed]
    [Google Scholar]
  8. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25: 125– 128 [CrossRef]
    [Google Scholar]
  9. Hugh R, Leifson E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol 1953; 66: 22– 26 [PubMed]
    [Google Scholar]
  10. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics New York: John Wiley and Sons; 1991; pp. 115– 148
    [Google Scholar]
  11. Uchida H, Hamana K, Miyazaki M, Yoshida T, Nogi Y. Parasphingopyxis lamellibrachiae gen. nov., sp. nov., isolated from a marine organism in Kagoshima Bay, Japan. Int J Syst Evol Microbiol 2012; 62: 2224– 2228 [Crossref]
    [Google Scholar]
  12. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215: 403– 410 [CrossRef] [PubMed]
    [Google Scholar]
  13. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613– 1617 [CrossRef] [PubMed]
    [Google Scholar]
  14. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25: 4876– 4882 [CrossRef] [PubMed]
    [Google Scholar]
  15. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406– 425 [PubMed]
    [Google Scholar]
  16. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20: 406– 416 [CrossRef]
    [Google Scholar]
  17. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17: 368– 376 [CrossRef] [PubMed]
    [Google Scholar]
  18. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28: 2731– 2739 [CrossRef] [PubMed]
    [Google Scholar]
  19. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolismvol. 3 New York: Academic Press; 1969; pp. 21– 132 [Crossref]
    [Google Scholar]
  20. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006; 33: 152– 155
    [Google Scholar]
  21. MIDI Sherlock, Microbial Identification System, Operating Manual, Version 3.0 Newark, DE: MIDI, Inc; 1999
    [Google Scholar]
  22. Nogi Y, Yoshizumi M, Miyazaki M. Thalassospira povalilytica sp. nov., a polyvinyl-alcohol-degrading marine bacterium. Int J Syst Evol Microbiol 2014; 64: 1149– 1153 [CrossRef] [PubMed]
    [Google Scholar]
  23. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
  24. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Method Microbiol 1987; 19: 161– 207 [Crossref]
    [Google Scholar]
  25. Lafay B, Ruimy R, de Traubenberg CR, Breittmayer V, Gauthier MJ et al. Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima. Int J Syst Bacteriol 1995; 45: 290– 296 [CrossRef] [PubMed]
    [Google Scholar]
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