1887

Abstract

Strain ANT-18, isolated from a water sample taken from the Longtoushan Spring in Taiwan, was characterized using a polyphasic taxonomic approach. Cells of strain ANT-18 were Gram-stain-negative, aerobic, motile by gliding rods that were surrounded by a thick capsule and it formed pink colonies. Growth occurred at 20–37 °C (optimum, 25 °C), at pH 6–8 (optimum, pH 7) and with 0–1 % NaCl (w/; optimum, 0 %). Phylogenetic analyses, based on 16S rRNA gene sequences, showed that strain ANT-18 belongs to the genus Hymenobacter and showed the highest levels of sequence similarity to Hymenobacter ocellatus Myx 2105 (97.8 %) and Hymenobacter paludis KBP-30 (97.7 %). Strain ANT-18 contained iso-C15 : 0, anteiso-C15 : 0, C18 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B) as the predominant fatty acids. The major isoprenoid quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, one uncharacterized aminolipid, two uncharacterized aminophospholipids, two uncharacterized glycolipids and seven uncharacterized lipids. The major polyamine was homospermidine. The genomic DNA G+C content of strain ANT-18 was 63.6 mol%. The DNA–DNA relatedness values of strain ANT-18 with respect to H. ocellatus Myx 2105 and H. paludis KBP-30 were less than 35 %. On the basis of the phylogenetic inference and phenotypic data, strain ANT-18 is recognized to be a representative of a novel species within the genus Hymenobacter. The name Hymenobacter gummosus sp. nov. is proposed, with strain ANT-18 (=BCRC 80966=LMG 29477=KCTC 52166) as the type strain.

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2017-10-06
2019-09-21
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